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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0003241,
umls-concept:C0019682,
umls-concept:C0019699,
umls-concept:C0021289,
umls-concept:C0030351,
umls-concept:C0180860,
umls-concept:C0370231,
umls-concept:C0443131,
umls-concept:C0581406,
umls-concept:C1511790,
umls-concept:C1512080,
umls-concept:C1522664,
umls-concept:C1546637,
umls-concept:C1550638,
umls-concept:C1704449,
umls-concept:C1704684,
umls-concept:C1858460,
umls-concept:C2827485
|
| pubmed:issue |
4
|
| pubmed:dateCreated |
1993-7-15
|
| pubmed:abstractText |
The testing of neonatal blood specimens dried on filter paper for maternal HIV antibodies, using an enzyme immunoassay (EIA) with confirmation of repeatedly reactive specimens by immunoblot (IB), was first described in 1987. It has been used to conduct large, unlinked, anonymous HIV seroprevalence surveys for surveillance of HIV in child-bearing women in several countries. We directly assessed the sensitivity and specificity of this combination of tests to detect maternal HIV antibodies.
|
| pubmed:keyword |
http://linkedlifedata.com/resource/pubmed/keyword/Acquired Immunodeficiency Syndrome,
http://linkedlifedata.com/resource/pubmed/keyword/Comparative Studies,
http://linkedlifedata.com/resource/pubmed/keyword/Developing Countries,
http://linkedlifedata.com/resource/pubmed/keyword/Diseases,
http://linkedlifedata.com/resource/pubmed/keyword/Examinations And Diagnoses,
http://linkedlifedata.com/resource/pubmed/keyword/Family And Household,
http://linkedlifedata.com/resource/pubmed/keyword/Family Characteristics,
http://linkedlifedata.com/resource/pubmed/keyword/Family Relationships,
http://linkedlifedata.com/resource/pubmed/keyword/Hematologic Tests,
http://linkedlifedata.com/resource/pubmed/keyword/Hiv Infections,
http://linkedlifedata.com/resource/pubmed/keyword/Hiv Serodiagnosis,
http://linkedlifedata.com/resource/pubmed/keyword/Laboratory Examinations And Diagnoses,
http://linkedlifedata.com/resource/pubmed/keyword/Laboratory Procedures,
http://linkedlifedata.com/resource/pubmed/keyword/Mothers,
http://linkedlifedata.com/resource/pubmed/keyword/PARENTS,
http://linkedlifedata.com/resource/pubmed/keyword/Research Methodology,
http://linkedlifedata.com/resource/pubmed/keyword/Research Report,
http://linkedlifedata.com/resource/pubmed/keyword/Studies,
http://linkedlifedata.com/resource/pubmed/keyword/Viral Diseases
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0269-9370
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
483-8
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| pubmed:dateRevised |
2004-11-17
|
| pubmed:otherAbstract |
PIP: Neonatal blood specimens dried on filter paper have been tested for maternal HIV antibodies in large, unlinked, anonymous HIV seroprevalence surveys toward the surveillance of HIV in child-bearing women in several countries. This study assesses the sensitivity and specificity of this combination of tests. The standard approach involves first testing the sample via an enzyme immunoassay (EIA), then confirming repeatedly reactive specimens through immunoblot (IB). To test this methodology, serum samples were obtained from mothers delivering at a major hospital in Kinshasa, Zaire, and screened with rapid assay HIVCHEK for antibody to HIV. Plasma from HIVCHEK-positive women and age-matched negative controls were then subjected to ELISA, with repeatedly reactive samples confirmed with Western blot. Whole blood was later obtained by heel-stick from each newborn 2 days after delivery, dried on filter paper, and tested by EIA and IB for confirmation. The serologic statuses of neonatal filter-paper specimens were then compared with those of corresponding maternal plasma. 100% sensitivity was achieved by testing neonatal filter-paper specimens with EIA and confirming with IB. The combination of tests also proved 99.6% specific for detecting maternal HIV antibodies; both results are at 95% confidence intervals. These results demonstrate that maternal HIV antibodies can therefore be detected accurately by testing neonatal blood dried on filter paper, using EIA, then confirming repeatedly reactive specimens via IB. This approach may help determine HIV seroprevalence in childbearing women in countries with neonatal screening programs or where serum or plasma is difficult to obtain.
|
| pubmed:meshHeading |
pubmed-meshheading:8507414-Democratic Republic of the Congo,
pubmed-meshheading:8507414-Female,
pubmed-meshheading:8507414-HIV Antibodies,
pubmed-meshheading:8507414-HIV Infections,
pubmed-meshheading:8507414-HIV Seroprevalence,
pubmed-meshheading:8507414-Humans,
pubmed-meshheading:8507414-Immunoenzyme Techniques,
pubmed-meshheading:8507414-Infant, Newborn,
pubmed-meshheading:8507414-Maternal-Fetal Exchange,
pubmed-meshheading:8507414-Pregnancy,
pubmed-meshheading:8507414-Pregnancy Complications, Infectious,
pubmed-meshheading:8507414-Sensitivity and Specificity
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Accurate detection of maternal antibodies to HIV in newborn whole blood dried on filter paper.
|
| pubmed:affiliation |
Division of HIV/AIDS, Centers for Disease Control and Prevention, Atlanta, GA 30333.
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| pubmed:publicationType |
Journal Article
|