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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1993-7-2
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pubmed:databankReference |
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pubmed:abstractText |
Human 1,25-dihydroxyvitamin D3 24-hydroxylase cDNA clones were isolated from an HL-60 cell cDNA library by using a reverse transcription/polymerase chain reaction-generated human cDNA probe. The 24-hydroxylase cDNA consists of a 1539-bp open reading frame encoding a 513-amino acid polypeptide. Protein sequence analysis shows that the human 24-hydroxylase is 90% homologous (82% identical) to that of the rat, with 100% homology in the 21-amino acid heme-binding region. Northern blot analysis showed that the 24-hydroxylase cDNA probe hybridized to a 3.4-kb mRNA species. Treatment of HL-60 cells with 0.1 microM 1,25-dihydroxyvitamin D3 for 24 hr produced a 30-fold increase in the 24-hydroxylase mRNA level. This result is consistent with previous studies in the same cell line, in which 24-hydroxylase activity was elevated to a maximum in 24 hr by a similar treatment with 1,25-dihydroxyvitamin D3. To verify the identity of these isolated cDNA clones, two polymerase chain reaction-amplified human 24-hydroxylase cDNA fragments containing the entire coding region were used to produce 24-hydroxylase enzyme activity in two genetic expression systems. Transient levels of 24-hydroxylase activity were measured in transfected mammalian COS-1 cells and in recombinant baculovirus-infected Spodoptera frugiperda (Sf21) insect cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-1200639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-1846519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-1903203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-1991512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-2178322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-2506177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-271272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-2722778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-2819041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-2842897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-3000754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-3280376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-3459588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-6311080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-6315339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-6322160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-6332645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-6581807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-68913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-6948604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506296-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4543-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8506296-Amino Acid Sequence,
pubmed-meshheading:8506296-Animals,
pubmed-meshheading:8506296-Base Sequence,
pubmed-meshheading:8506296-Cell Line,
pubmed-meshheading:8506296-Cercopithecus aethiops,
pubmed-meshheading:8506296-Cloning, Molecular,
pubmed-meshheading:8506296-Cytochrome P-450 Enzyme System,
pubmed-meshheading:8506296-DNA,
pubmed-meshheading:8506296-Gene Expression,
pubmed-meshheading:8506296-Humans,
pubmed-meshheading:8506296-Molecular Sequence Data,
pubmed-meshheading:8506296-Moths,
pubmed-meshheading:8506296-RNA, Messenger,
pubmed-meshheading:8506296-Rats,
pubmed-meshheading:8506296-Sequence Alignment,
pubmed-meshheading:8506296-Steroid Hydroxylases,
pubmed-meshheading:8506296-Transfection,
pubmed-meshheading:8506296-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
Isolation and expression of human 1,25-dihydroxyvitamin D3 24-hydroxylase cDNA.
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pubmed:affiliation |
Department of Biochemistry, College of Agriculture and Life Science, University of Wisconsin-Madison 53706.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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