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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1993-7-8
|
| pubmed:abstractText |
Toxic shock syndrome toxin (TSST-1), a 22-kDa exotoxin secreted by Staphylococcus aureus, can act as a nominal antigen and induce proliferation and immunoglobulin secretion in human B-cells. The purpose of the present studies was to examine the effect of 7,12-dimethylbenz[a]anthracene (DMBA), a well-characterized immunosuppressant of both cell-mediated and humoral immunity in murine lymphocytes, upon the mixed lymphocyte reaction (MLR) and TSST-1-induced immune responses in human lymphocytes. The MLR, using human tonsillar lymphocytes (HTL) from four different donors, was inhibited in a dose-dependent manner from 1 to 100 microM. The IC50 for the suppression of the MLR ranged from 10 to 40 microM. TSST-1 is a potent stimulator of T-cells bearing specific VB regions on the T-cell receptor (TCR). In contrast with the results from the MLR, DMBA inhibited TSST-1-induced T-cell proliferation only at 100 microM in HTL. A similar profile of activity was determined with splenic T-cells from a single donor. TSST-1 has also been demonstrated to induce specific B-cell proliferation and differentiation in the presence of irradiated T-cells. TSST-1-induced B-cell proliferation was only consistently and markedly inhibited by DMBA at 100 microM in tonsillar and splenic lymphocytes. In contrast, TSST-1-induced B-cell differentiation, as manifested by IgM and IgG secretion, was inhibited in a dose-dependent manner from 1 to 100 microM DMBA in B-cells from human tonsils and spleens.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/9,10-Dimethyl-1,2-benzanthracene,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Enterotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Superantigens,
http://linkedlifedata.com/resource/pubmed/chemical/enterotoxin F, Staphylococcal
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0272-0590
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
20
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
280-7
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8504901-9,10-Dimethyl-1,2-benzanthracene,
pubmed-meshheading:8504901-Adolescent,
pubmed-meshheading:8504901-Adult,
pubmed-meshheading:8504901-B-Lymphocytes,
pubmed-meshheading:8504901-Bacterial Toxins,
pubmed-meshheading:8504901-Cell Division,
pubmed-meshheading:8504901-Child,
pubmed-meshheading:8504901-Child, Preschool,
pubmed-meshheading:8504901-Enterotoxins,
pubmed-meshheading:8504901-Female,
pubmed-meshheading:8504901-Humans,
pubmed-meshheading:8504901-Immunoglobulin G,
pubmed-meshheading:8504901-Lymphocyte Activation,
pubmed-meshheading:8504901-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:8504901-Male,
pubmed-meshheading:8504901-Palatine Tonsil,
pubmed-meshheading:8504901-Spleen,
pubmed-meshheading:8504901-Staphylococcus aureus,
pubmed-meshheading:8504901-Superantigens,
pubmed-meshheading:8504901-T-Lymphocytes
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Effects of 7,12-dimethylbenz[a]anthracene on the superantigen toxic shock syndrome toxin (TSST-1)-induced proliferation and antibody secretion by human lymphocytes.
|
| pubmed:affiliation |
Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|