Linked Life Data

8504288

Source:http://linkedlifedata.com/resource/pubmed/id/8504288

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-7-8
pubmed:abstractText
62Cu(T1/2 = 9.8 min) is a generator-produced positron-emitting radionuclide with a half-life amenable to blood-pool imaging with PET. Three bifunctional chelates [cyclic anhydride of diethylenetriaminepentaacetic acid (cDTPAA), 6-bromoacetamidobenzyl-1,4,8,11-tetraazacyclotetradecane-N,N ',N", N"'-tetraacetic acid (BAT), and p-carboxyethylphenylglyoxal-bis-(4N-methyl-thiosemicarbazone (CE-DTS)] were conjugated to HSA and labeled with 67Cu. The labeling efficiency of 67Cu-DTS-HSA was > 90%, whereas the labeling yields of 67Cu-DTPA-HSA and 67Cu-benzyl-TETA-HSA were less than 70%. Blood clearance and biodistribution of these three 67Cu-labeled conjugates were determined in rats. Of the three 67Cu-labeled bifunctional chelate-HSA conjugates, 67Cu-benzyl-TETA-HSA remained in the blood pool the longest, achieving stable blood levels at times longer than 24 h post-injection. The 67Cu radioactivity cleared the blood within 60 min post-injection of 67Cu-DTS-HSA, and within 10 min after administration of 67Cu-DTPA-HSA, indicating the dissociation of Cu2+ from these conjugates. Copper-labeled DTS-HSA achieved stable blood concentrations for at least 30 min post-injection and was therefore evaluated as a vascular imaging agent. DTS-HSA and benzyl-TETA-HSA were labeled with 62Cu and administered to a dog for blood-pool imaging using PET. Images were nearly identical to an image taken after administration of C15O. Because of the high labeling efficiency, DTS-HSA can be labeled with 62Cu without purification, making it more practical than 62Cu-benzyl-TETA-HSA as a blood-pool imaging agent.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0969-8051
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
461-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8504288-Animals, pubmed-meshheading:8504288-Chelating Agents, pubmed-meshheading:8504288-Copper, pubmed-meshheading:8504288-Copper Radioisotopes, pubmed-meshheading:8504288-Coronary Vessels, pubmed-meshheading:8504288-Cross-Linking Reagents, pubmed-meshheading:8504288-Diagnostic Imaging, pubmed-meshheading:8504288-Dogs, pubmed-meshheading:8504288-Drug Stability, pubmed-meshheading:8504288-Female, pubmed-meshheading:8504288-Heart, pubmed-meshheading:8504288-Heterocyclic Compounds, pubmed-meshheading:8504288-Male, pubmed-meshheading:8504288-Organometallic Compounds, pubmed-meshheading:8504288-Pentetic Acid, pubmed-meshheading:8504288-Rats, pubmed-meshheading:8504288-Rats, Sprague-Dawley, pubmed-meshheading:8504288-Serum Albumin, pubmed-meshheading:8504288-Thiosemicarbazones, pubmed-meshheading:8504288-Tissue Distribution, pubmed-meshheading:8504288-Tomography, Emission-Computed
pubmed:year
1993
pubmed:articleTitle
Evaluation of copper-labeled bifunctional chelate-albumin conjugates for blood pool imaging.
pubmed:affiliation
Mallinckrodt Institute of Radiology, Washington University Medical School, St Louis, MO 63110.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S.