Linked Life Data

8503191

Source:http://linkedlifedata.com/resource/pubmed/id/8503191

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-6-25
pubmed:databankReference
pubmed:abstractText
Previous studies of HIV-1 replication in chimpanzee lymphocytes have been limited to a small number of virus isolates which generally replicated poorly and without cytopathic effect. Here, we describe an HIV-1 provirus (SG3), cloned as a single proviral unit, which replicates more efficiently in chimpanzee than in human lymphocytes, resulting in syncytium formation and cell death. This provirus also replicates efficiently and with extraordinary cytopathic effect in immortalized human T-cell lines. The SG3 genome was completely sequenced and found to contain all genes typical of HIV-1 with the exception of vpu. Phylogenetically, SG3 is representative of North American/European strains of HIV-1 and shows no greater similarity to SIVCPZ in its envelope sequence than do other HIV-1 strains. Pilot studies indicate that SG3 can infect and replicate in chimpanzees and SCID-hu mice in vivo following intravenous or intrathymic inoculation. The SG3 proviral clone, because of its replication efficiency in human and chimpanzee cells and its complete molecular description, represents a new virologic reagent for the coordinated molecular analysis of HIV-1 replication and pathogenesis in relevant animal model systems.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
194
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
858-64
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
A molecular clone of HIV-1 tropic and cytopathic for human and chimpanzee lymphocytes.
pubmed:affiliation
Department of Medicine, University of Alabama, Birmingham 35294.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.