Linked Life Data

8501395

Source:http://linkedlifedata.com/resource/pubmed/id/8501395

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-6-29
pubmed:abstractText
Temporally distinct groups of cytokine expression was observed by reverse transcription-polymerase chain reaction assay, in situ hybridization, and immunohistochemistry in the livers of Listeria monocytogenes-infected mice. One group consisted of interferon-gamma (IFN-gamma), tumor necrosis factor alpha (TNF-alpha), and interleukin-10 (IL-10), for which mRNAs were induced within 1 day after challenge. A second group consisted of IL-2 and IL-4, for which mRNA was strongly expressed at 1 day but then suppressed at 3 days into the infection. Granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-1 alpha, and IL-6 mRNA constituted a third group, which was increased at 3 days after challenge. Distributions of cytokine mRNA-expressing cells in the liver was observed by in situ hybridization. Cells expressing TNF-alpha and IL-1 alpha mRNA were present throughout liver granulomas, whereas cells that expressed IFN-gamma mRNA were observed mostly along the periphery of granulomas. Cells expressing IL-2, IL-4, IL-6, IL-10, and GM-CSF mRNA were distributed principally in the hepatic sinuses. Cells expressing IL-10 mRNA increased in number early in the infection when L. monocytogenes was multiplying in the liver. We conclude that cytokine mRNA expression during the early phases of L. monocytogenes infection in mice is temporally regulated and that IFN-gamma, TNF-alpha, and IL-1 alpha are expressed by cells associated with hepatic granulomas.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0741-5400
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
525-31
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Cytokine mRNA expression in livers of mice infected with Listeria monocytogenes.
pubmed:affiliation
Department of Pathobiological Sciences, University of Wisconsin School of Veterinary Medicine, Madison 53706.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.