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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1993-6-25
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pubmed:abstractText |
Alpha factor induces arrest of yeast a cells in G1 and transcription of genes involved in mating. Prior work indicates that FUS3, a member of the MAP kinase family, and FAR1, whose molecular activity is unknown, contribute to cell cycle arrest by inhibiting G1 cyclins. Here we show that FAR1 is a substrate for FUS3 and that this phosphorylation regulates association of FAR1 with CDC28-CLN2 kinase. We show also that FAR1 is phosphorylated in vitro by the CDC28-CLN2 complex and in vivo in a CDC28-dependent manner. Mutational analysis of FAR1 reveals a correlation between its ability to associate with CDC28-CLN2 and to arrest the cell cycle. These results suggest that FAR1 protein is the link between the signaling pathway and the cell cycle machinery.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDC28 Protein Kinase, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/FUS3 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/mating factor
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
747-60
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8500168-Amino Acid Sequence,
pubmed-meshheading:8500168-Base Sequence,
pubmed-meshheading:8500168-CDC28 Protein Kinase, S cerevisiae,
pubmed-meshheading:8500168-Cell Cycle,
pubmed-meshheading:8500168-Cell Cycle Proteins,
pubmed-meshheading:8500168-Cyclin-Dependent Kinase Inhibitor Proteins,
pubmed-meshheading:8500168-DNA Mutational Analysis,
pubmed-meshheading:8500168-Fungal Proteins,
pubmed-meshheading:8500168-Mitogen-Activated Protein Kinases,
pubmed-meshheading:8500168-Molecular Sequence Data,
pubmed-meshheading:8500168-Peptides,
pubmed-meshheading:8500168-Phosphorylation,
pubmed-meshheading:8500168-Precipitin Tests,
pubmed-meshheading:8500168-Protein Binding,
pubmed-meshheading:8500168-Repressor Proteins,
pubmed-meshheading:8500168-Saccharomyces cerevisiae,
pubmed-meshheading:8500168-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:8500168-Signal Transduction
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pubmed:year |
1993
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pubmed:articleTitle |
FAR1 links the signal transduction pathway to the cell cycle machinery in yeast.
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pubmed:affiliation |
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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