Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1993-7-1
|
| pubmed:abstractText |
We have used a single-cell based polymerase chain reaction (PCR) amplification technique to examine the gene expression pattern in single Hodgkin's and Reed-Sternberg (H&RS) cells from seven patients with Hodgkin's disease. Single cells were isolated from lymph nodes obtained at diagnosis (5 of 7 patients) or in first or second relapse (2 of 7 patients). Gene expression was examined by hybridization to a panel of 22 cDNA probes. Forty-nine H&RS cells (and 23 CD3+ or CD20+ lymphocytes as controls) from four patients with nodular sclerosing Hodgkin's disease (HD) and one patient each with lymphocyte predominant and mixed-cellularity HD were successfully analyzed by PCR. This analysis provides evidence that single H&RS cells can coexpress genes characteristic of several hematopoietic lineages (monocytes and lymphocytes). Genes characteristic of activated lymphoid cells are expressed in most H&RS cells. Heterogeneity of expression for certain genes between different cases was found and may eventually define molecular subgroups of HD. These findings indicate that H&RS cells of HD resemble activated hematopoietic cells. Phenotypically similar cells from different cases exhibit characteristic molecular differences. In one patient, 5 of 7 single RS cells showed identical p53 cDNA mutations at codon 246 on specific reverse transcriptase [RT]-PCR and sequencing of exons 5 through 8. The novel experimental approach may provide a valuable tool for understanding the molecular events in newly diagnosed Hodgkin's disease and progression of the disease.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
81
|
| pubmed:geneSymbol |
CD2,
CD20,
CD30-7,
CD34,
CD4,
GATA 3,
IL-2 R&bgr;,
IL-9,
TCR&bgr;,
TNF &bgr;,
c-fes,
c-fms,
c-myc,
fps,
fyn,
lck
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
3097-115
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8499644-Adolescent,
pubmed-meshheading:8499644-Adult,
pubmed-meshheading:8499644-Base Sequence,
pubmed-meshheading:8499644-Cloning, Molecular,
pubmed-meshheading:8499644-Cytokines,
pubmed-meshheading:8499644-Female,
pubmed-meshheading:8499644-Gene Expression,
pubmed-meshheading:8499644-Genes, p53,
pubmed-meshheading:8499644-Hodgkin Disease,
pubmed-meshheading:8499644-Humans,
pubmed-meshheading:8499644-Lymph Nodes,
pubmed-meshheading:8499644-Male,
pubmed-meshheading:8499644-Middle Aged,
pubmed-meshheading:8499644-Molecular Sequence Data,
pubmed-meshheading:8499644-Mutation,
pubmed-meshheading:8499644-Oligodeoxyribonucleotides,
pubmed-meshheading:8499644-Polymerase Chain Reaction,
pubmed-meshheading:8499644-RNA, Messenger,
pubmed-meshheading:8499644-Reed-Sternberg Cells
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Single-cell analysis of Hodgkin and Reed-Sternberg cells: molecular heterogeneity of gene expression and p53 mutations.
|
| pubmed:affiliation |
Division of Molecular and Cellular Biology, Ontario Cancer Institute, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|