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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1993-6-23
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pubmed:abstractText |
The Cln3 cyclin homolog of Saccharomyces cerevisiae functions to promote cell cycle START for only a short time following its synthesis. Cln3 protein is highly unstable and is stabilized by C-terminal truncation. Cln3 binds to Cdc28, a protein kinase catalytic subunit essential for cell cycle START, and Cln3 instability requires Cdc28 activity. The long functional lifetime and the hyperactivity of C-terminally truncated Cln3 (Cln3-2) relative to those of full-length Cln3 are affected by mutations in CDC28: the functional lifetime of Cln3-2 is drastically reduced by the cdc28-13 mutation at the permissive temperature, and the cdc28-4 mutation at the permissive temperature completely blocks the function of Cln3-2 while only partially reducing the function of full-length Cln3. Thus, sequences in the C-terminal third of Cln3 might help stabilize functional Cdc28-Cln3 association, as well as decreasing the lifetime of the Cln3 protein. These and other results strongly support the idea that Cln proteins function to activate Cdc28 at START.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1316273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1652371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1653904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1704128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1783298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1827756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1827757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1828291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1829983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1833066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1833067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1833068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1836192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1840248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-1846030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2040016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2142620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2143810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2147225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2147872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2149647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2254321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2561422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2569741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2574592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2574633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2847913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2876518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-2907481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-3062366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-3290211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-4587263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-6204768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8497251-7021310
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:geneSymbol |
CDC28,
CLIN3-2,
CLN3,
cdc28-13,
cdc28-4,
daf1-2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3266-71
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8497251-CDC28 Protein Kinase, S cerevisiae,
pubmed-meshheading:8497251-Cell Cycle,
pubmed-meshheading:8497251-Cyclins,
pubmed-meshheading:8497251-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8497251-Enzyme Activation,
pubmed-meshheading:8497251-Fungal Proteins,
pubmed-meshheading:8497251-Genes, Fungal,
pubmed-meshheading:8497251-Immunoblotting,
pubmed-meshheading:8497251-Mutagenesis,
pubmed-meshheading:8497251-Plasmids,
pubmed-meshheading:8497251-Saccharomyces cerevisiae,
pubmed-meshheading:8497251-Saccharomyces cerevisiae Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
The yeast Cln3 protein is an unstable activator of Cdc28.
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pubmed:affiliation |
Rockefeller University, New York, New York 10021.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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