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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1993-6-22
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pubmed:abstractText |
DRK1 is a cloned K+ channel from rat brain with consensus sites for protein kinase-dependent phosphorylation that might be expected to be functionally regulated by phosphorylation. 2,3-Butane-dione-monoxime (BDM) chemically removes phosphate groups from many proteins, and its action on DRK1 channels was examined after expression of DRK1 cRNA in Xenopus oocytes. In two-microelectrode voltage-clamp experiments, the application of BDM to the bath inhibited DRK1 current (ki = 16.6 mM, H = 0.96) rapidly and reversibly, with a time course similar to the time course of solution change within the bath. DRK1 current was inhibited at all potentials; the time course of current activation, deactivation and inactivation were unaffected by BDM. In inside-out patch-clamp experiments, the application of BDM to the cytoplasmic surface similarly inhibited channel activity rapidly and reversibly (ki = 10.7 mM, H = 1.01) in the absence of rephosphorylating substrates. These results are inconsistent with a phosphatase effect, because such an effect should be irreversible in cell-free, ATP-free patches. Instead, the results suggest that BDM can inhibit DRK1 channels directly from inside or outside of the membrane.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-3565
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
265
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1011-6
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8496800-Animals,
pubmed-meshheading:8496800-Biological Transport,
pubmed-meshheading:8496800-Cloning, Molecular,
pubmed-meshheading:8496800-Diacetyl,
pubmed-meshheading:8496800-Diffusion,
pubmed-meshheading:8496800-Membrane Potentials,
pubmed-meshheading:8496800-Oocytes,
pubmed-meshheading:8496800-Potassium Channels,
pubmed-meshheading:8496800-Rats,
pubmed-meshheading:8496800-Xenopus
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pubmed:year |
1993
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pubmed:articleTitle |
2,3-Butanedione monoxime (BDM) inhibition of delayed rectifier DRK1 (Kv2.1) potassium channels expressed in Xenopus oocytes.
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pubmed:affiliation |
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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