Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1993-6-22
|
| pubmed:abstractText |
FBL-3N is an MHC class II Ag+ variant line that was obtained spontaneously during maintenance of Friend virus-induced leukemia FBL-3 in an athymic C57BL/6 (B6) mouse. Inocula of FBL-3N, but not the parental FBL-3 tumor, regressed after initial growth in CD8-depleted, syngeneic B6 mice. The cellular mechanisms by which FBL-3N was rejected in these mice were investigated in this study. We demonstrated that CTL with both CD4+ and CD4-CD8-TCR-alpha beta phenotypes were generated in mixed lymphocyte tumor cell culture spleen cells obtained from CD8-depleted B6 mice that had rejected FBL-3N by in vitro stimulation with mitomycin C-treated FBL-3N. After adoptive transfer of these CTL that were generated in vitro into athymic B6 mice, challenge with the FBL-3N tumor resulted in tumor regression after its initial growth. Thus, CD4+ and CD4-CD8-TCR-alpha beta CTL mediated rejection of the FBL-3N tumor in CD8-depleted B6 mice. Furthermore, the findings that depletion of B6 mice of CD4+ cells in addition to CD8+ cells abrogated the rejection of FBL-3N and generation of CTL in mixed lymphocyte tumor cell culture spleen cells suggest that CD4+ cells were required not only as a source of CD4+ CTL, but also as helper cells for generation of CD4-CD8-TCR-alpha beta CTL. Tumor Ag recognition of CD4-CD8-TCR-alpha beta CTL was restricted to Db, like that of classical CD8+ CTL, but the restriction appeared to be less obligatory than that of CD8+ CTL.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
150
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4900-10
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8496592-Animals,
pubmed-meshheading:8496592-Antibody Specificity,
pubmed-meshheading:8496592-Antigens, CD4,
pubmed-meshheading:8496592-Antigens, CD8,
pubmed-meshheading:8496592-Binding Sites, Antibody,
pubmed-meshheading:8496592-Clone Cells,
pubmed-meshheading:8496592-Cytotoxicity, Immunologic,
pubmed-meshheading:8496592-Flow Cytometry,
pubmed-meshheading:8496592-Friend murine leukemia virus,
pubmed-meshheading:8496592-Genetic Variation,
pubmed-meshheading:8496592-Graft Rejection,
pubmed-meshheading:8496592-Histocompatibility Antigens Class II,
pubmed-meshheading:8496592-Immunotherapy, Adoptive,
pubmed-meshheading:8496592-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:8496592-Lymphocyte Depletion,
pubmed-meshheading:8496592-Mice,
pubmed-meshheading:8496592-Mice, Inbred C57BL,
pubmed-meshheading:8496592-Neoplasm Transplantation,
pubmed-meshheading:8496592-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:8496592-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:8496592-Tumor Cells, Cultured
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Rejection of an IA+ variant line of FBL-3 leukemia by cytotoxic T lymphocytes with CD4+ and CD4-CD8- T cell receptor-alpha beta phenotypes generated in CD8-depleted C57BL/6 mice.
|
| pubmed:affiliation |
Department of Periodontology, Nagasaki University School of Dentistry, Japan.
|
| pubmed:publicationType |
Journal Article
|