Linked Life Data

8495796

Source:http://linkedlifedata.com/resource/pubmed/id/8495796

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-6-18
pubmed:abstractText
A cDNA library was prepared from quiescent guinea-pig endometrial glandular epithelial cells stimulated for 2 h with estradiol-17 beta (E2) in the presence of cycloheximide. It was screened by differential hybridization for estrogen-regulated sequences. Six recombinants containing E2-regulated sequences were identified. One of them, called gec1 was then characterized by Northern blot hybridization. The gec1 mRNA was 1,800 bases in size. A 2-fold increase in the gec1 mRNA level was achieved at 120 min after E2 treatment. The E2 action on gec1 gene required the presence of cycloheximide. The cloned gec1 cDNA was 1 kb in size. The sequence so far determined did not show similarity with well characterized genes. This is the first report on a cloned cDNA probe of early estrogen-induced mRNA in a primary culture of endometrial epithelial cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:volume
90
pubmed:geneSymbol
c-fos, gec1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
R17-21
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Identification and characterization of an early estrogen-regulated RNA in cultured guinea-pig endometrial cells.
pubmed:affiliation
INSERM U 198, Besançon, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't