| pubmed-article:8495484 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8495484 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:8495484 | lifeskim:mentions | umls-concept:C0040669 | lld:lifeskim |
| pubmed-article:8495484 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8495484 | lifeskim:mentions | umls-concept:C0026473 | lld:lifeskim |
| pubmed-article:8495484 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:8495484 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
| pubmed-article:8495484 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8495484 | pubmed:dateCreated | 1993-6-22 | lld:pubmed |
| pubmed-article:8495484 | pubmed:abstractText | To investigate the expression of human immunodeficiency virus (HIV) genes in human monocytes, a DNA transfection system was developed and characterized using cultured primary monocytes. Monocytes that were cultured 6-7 days in an adherent monolayer were efficiently recovered and transfected by electroporation with an expression vector containing the Escherichia coli lacZ gene under control of the cytomegalovirus immediate-early promoter. Successful transfection was detected by expression of beta-galactosidase activity and by histochemical staining for beta-galactosidase in cells that were allowed to readhere to plastic following transfection. Over 30% of the surviving adherent monocytes expressed the transfected beta-galactosidase gene. In the same manner, monocytes were transfected with HIV provirus clones pIIIB and pIIB/PB. The provirus pIIIB/PB differs from pIIIB only in that it contains a small sequence from the env gene of a macrophage tropic HIV-1. Virus derived from pIIIB will not replicate in monocytes whereas virus derived from pIIIB/PB will. Monocytes transfected with either provirus DNA expressed high levels of p24 antigen within 1 day of transfection, and cell-free supernatants contained virus that was infectious for T cells. In contrast, only supernatants from pIIIB/PB transfections contained virus capable of infecting monocytes. Thus, proviral DNA of T cell tropic HIV efficiently completes the retroviral life cycle in monocytes in a manner indistinguishable from that of macrophage tropic HIV, and progeny virus retain their T cell tropism. | lld:pubmed |
| pubmed-article:8495484 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8495484 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8495484 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8495484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8495484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8495484 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8495484 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8495484 | pubmed:issn | 0008-8749 | lld:pubmed |
| pubmed-article:8495484 | pubmed:author | pubmed-author:MeltzerM SMS | lld:pubmed |
| pubmed-article:8495484 | pubmed:author | pubmed-author:WeirJ PJP | lld:pubmed |
| pubmed-article:8495484 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8495484 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:8495484 | pubmed:volume | 148 | lld:pubmed |
| pubmed-article:8495484 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8495484 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8495484 | pubmed:pagination | 157-65 | lld:pubmed |
| pubmed-article:8495484 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:8495484 | pubmed:meshHeading | pubmed-meshheading:8495484-... | lld:pubmed |
| pubmed-article:8495484 | pubmed:meshHeading | pubmed-meshheading:8495484-... | lld:pubmed |
| pubmed-article:8495484 | pubmed:meshHeading | pubmed-meshheading:8495484-... | lld:pubmed |
| pubmed-article:8495484 | pubmed:meshHeading | pubmed-meshheading:8495484-... | lld:pubmed |
| pubmed-article:8495484 | pubmed:meshHeading | pubmed-meshheading:8495484-... | lld:pubmed |
| pubmed-article:8495484 | pubmed:meshHeading | pubmed-meshheading:8495484-... | lld:pubmed |
| pubmed-article:8495484 | pubmed:meshHeading | pubmed-meshheading:8495484-... | lld:pubmed |
| pubmed-article:8495484 | pubmed:meshHeading | pubmed-meshheading:8495484-... | lld:pubmed |
| pubmed-article:8495484 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8495484 | pubmed:articleTitle | Transfection of human immunodeficiency virus type 1 proviral DNA into primary human monocytes. | lld:pubmed |
| pubmed-article:8495484 | pubmed:affiliation | Department of Cellular Immunology, Walter Reed Army Institute of Research, Washington, DC 20307-5100. | lld:pubmed |
| pubmed-article:8495484 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8495484 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8495484 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8495484 | lld:pubmed |
