Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-6-23
pubmed:abstractText
Intracellular calcium ion ([Ca2+]i) transients were measured in single rat ventricular myocytes with the fluorescent indicator furaptra. Cells were voltage clamped with a single patch electrode containing the K+ salt of furaptra and fluorescence at 500 nm was measured during illumination with 350 and 370 nm light. Depolarizing voltage-clamp pulses elicited [Ca2+]-dependent fluorescent transients in 30 of 33 cells tested. The peak change in [Ca2+]i elicited by 50-ms depolarizations from -70 to +10 mV was 1.52 +/- 0.25 microM (mean +/- SEM, n = 7). The size of the [Ca2+]i transient increased in response to 10 microM isoproterenol, prolongation of the depolarization, and increasing pipette [Na+]. Because furaptra is sensitive to Ca2+ and Mg2+, changes in [Mg2+]i during the [Ca2+]i transient could not be measured. Instead, a single-compartment model was developed to simulate changes in [Mg2+] during [Ca2+] transients. The simulations predicted that a 2 microM [Ca2+] transient was accompanied by a slow increase in [Mg2+] (14-29 microM), which became larger as basal [Mg2+] increased (0.5-2.0 mM). The [Mg2+] transient reached a peak approximately 1 s after the peak of the [Ca2+] transient with the slow changes in [Mg2+] dominated by competition at the Ca2+/Mg2+ sites of Troponin. These changes in [Mg2+], however, were so small and slow that they were unlikely to affect the furaptra fluorescence signal at the peak of the [Ca2+]i transient. The [Ca2+]i transient reported by furaptra appears to be larger than that reported by other Ca2+ indicators; however, we conclude this larger transient is at least as accurate as [Ca2+]i transients reported by the other indicators.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-1420876, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-1653321, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-1770453, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-1892868, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2016581, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2042963, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2110515, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2275965, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2333986, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2335025, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2432238, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2446391, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2736729, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2747526, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2782433, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2786550, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2923192, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-2925624, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-307184, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-3113491, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-3115258, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-3158652, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-3185015, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-3194404, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-3266079, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-3267019, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-3395664, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-3505361, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-3714452, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-3838314, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-533865, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-6092625, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-6270629, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-6325589, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-6449512, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-6655593, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-6713605, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-6733242, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-6758036, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-6984069, http://linkedlifedata.com/resource/pubmed/commentcorrection/8494988-7195747
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1331-43
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Ca transients in cardiac myocytes measured with a low affinity fluorescent indicator, furaptra.
pubmed:affiliation
Bockus Research Institute, Graduate Hospital, Philadelphia, Pennsylvania 19146.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't