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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
13
|
| pubmed:dateCreated |
1993-6-4
|
| pubmed:databankReference | |
| pubmed:abstractText |
The structural protein ankyrin functions in red blood cells to link the spectrin-based membrane skeleton to the plasma membrane. Ankyrin proteins are now known to occur in most cell types, and two distinct ankyrin genes have been identified (erythroid (Ank-1) and brain (Ank-2)). We have characterized transcripts of the mouse erythroid ankyrin gene by cDNA cloning and DNA sequencing. Ank-1 transcripts of 7.5 and 9.0 kilobases are found in erythroid tissues, and a 9.0-kilobase transcript is found in cerebellum. RNA hybridization blot analysis of 13 additional mouse tissues has detected four novel Ank-1 transcripts (5.0, 3.5, 2.0, and 1.6 kilobases in size). Sequencing of Ank-1 cDNA clones isolated from mouse reticulocyte, spleen, and cerebellar libraries has identified (i) multiple 5' ends that indicate possible multiple promoters; (ii) alternative polyadenylation sites that probably account for the 7.5- and 9.0-kilobase size difference; (iii) a variety of small insertions and deletions that could produce transcripts (and ultimately proteins) of nearly identical size, but different functions; and (iv) clones with large deletions of coding sequence that account for the smaller transcripts seen in spleen, skeletal muscle, and heart.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
268
|
| pubmed:geneSymbol |
Ank-1,
Ank-2
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
9533-40
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8486643-Amino Acid Sequence,
pubmed-meshheading:8486643-Animals,
pubmed-meshheading:8486643-Ankyrins,
pubmed-meshheading:8486643-Base Sequence,
pubmed-meshheading:8486643-Cerebellum,
pubmed-meshheading:8486643-Cloning, Molecular,
pubmed-meshheading:8486643-DNA,
pubmed-meshheading:8486643-DNA Probes,
pubmed-meshheading:8486643-Exons,
pubmed-meshheading:8486643-Gene Library,
pubmed-meshheading:8486643-Genetic Variation,
pubmed-meshheading:8486643-Mice,
pubmed-meshheading:8486643-Mice, Inbred C57BL,
pubmed-meshheading:8486643-Molecular Sequence Data,
pubmed-meshheading:8486643-Organ Specificity,
pubmed-meshheading:8486643-Polymerase Chain Reaction,
pubmed-meshheading:8486643-Restriction Mapping,
pubmed-meshheading:8486643-Reticulocytes,
pubmed-meshheading:8486643-Sequence Homology, Amino Acid,
pubmed-meshheading:8486643-Spleen,
pubmed-meshheading:8486643-Transcription, Genetic
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Complex patterns of sequence variation and multiple 5' and 3' ends are found among transcripts of the erythroid ankyrin gene.
|
| pubmed:affiliation |
Jackson Laboratory, Bar Harbor, Maine 04609.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|