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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-6-10
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pubmed:abstractText |
In Salmonella typhimurium the metE and metR promoters overlap and are divergently transcribed. Three tandem repeats of an 8 bp sequence defined previously as the metE operator site for MetJ-mediated repression also overlap the -35 region of the metR promoter. Starting with a metE-lacZ.metR-galK double gene fusion, site-directed mutagenesis was used to change nucleotides in each of the repeat units from the consensus sequence. Each mutation, along with the wild-type metE-lacZ.metR-galK gene fusion, was cloned into phage lambda gt2. Regulation of the metE and metR genes was examined by measuring beta-galactosidase and galactokinase levels in Escherichia coli strains lysogenized with phage carrying the wild-type and mutant fusions. Mutations in each of the 8 bp repeat units disrupt MetJ-mediated repression for both the metE-lacZ and metR-galK gene fusions, suggesting that the metE and metR genes share a common operator site for the MetJ repressor.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5-Methyltetrahydrofolate-Homocystein...,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Galactokinase,
http://linkedlifedata.com/resource/pubmed/chemical/MetR protein, Bacteria,
http://linkedlifedata.com/resource/pubmed/chemical/Methyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase,
http://linkedlifedata.com/resource/pubmed/chemical/metE protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/methionine repressor protein...,
http://linkedlifedata.com/resource/pubmed/chemical/methionine repressor protein, E coli
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0378-1097
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
108
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pubmed:geneSymbol |
galK,
lacZ,
metE,
metJ,
metR
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
145-50
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8486240-5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase,
pubmed-meshheading:8486240-Bacterial Proteins,
pubmed-meshheading:8486240-Base Sequence,
pubmed-meshheading:8486240-Enzyme Repression,
pubmed-meshheading:8486240-Escherichia coli Proteins,
pubmed-meshheading:8486240-Galactokinase,
pubmed-meshheading:8486240-Gene Expression Regulation, Bacterial,
pubmed-meshheading:8486240-Methyltransferases,
pubmed-meshheading:8486240-Molecular Sequence Data,
pubmed-meshheading:8486240-Mutagenesis, Site-Directed,
pubmed-meshheading:8486240-Operator Regions, Genetic,
pubmed-meshheading:8486240-Recombinant Fusion Proteins,
pubmed-meshheading:8486240-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:8486240-Repressor Proteins,
pubmed-meshheading:8486240-Salmonella typhimurium,
pubmed-meshheading:8486240-Trans-Activators,
pubmed-meshheading:8486240-beta-Galactosidase
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pubmed:year |
1993
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pubmed:articleTitle |
MetJ-mediated regulation of the Salmonella typhimurium metE and metR genes occurs through a common operator region.
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pubmed:affiliation |
Department of Microbiology, University of Iowa, Iowa City 52242.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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