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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1993-6-3
|
| pubmed:abstractText |
Polytransfused patients often develop platelet-reactive antibodies (PRAb). These give positive reactions in the platelet immunofluorescence test (PIFT) and may be either lymphocytotoxic (LCTAb) or platelet-specific antibodies (PSAb). The latter may be detected in the PIFT using chloroquine-treated platelets (Chl-PIFT) or by immunoblotting. Serial samples from 106 multiply transfused patients with bone marrow failure were screened by PIFT using a microplate method and flow-cytometric analysis. PSAb activity was confirmed by Chl-PIFT. In 45 (42%) of the patients studied PSAb were detected; 37 (35%) formed LCTAb and 19 (51%) had co-existent PSAb. Sera from 25 of 27 patients with a positive Chl-PIFT, retested by immunoblotting, recognised determinants of Mr 82-160 kD on whole platelets. A large group became sensitised to a component of Mr 105-115 kD reduced (99 kD non-reduced) with similar electrophoretic mobility to GPIIIa using a monoclonal anti-GPIIIa and two human polyclonal anti-HPA-1a sera; some also produced anti-GPIIb. The largest group recognised a determinant of Mr 80-83 kD, probably glycoprotein V (GPV). Three sera were immunoblotted against thrombin-treated platelets and the results confirmed GPV specificity.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0042-9007
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
64
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
167-70
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8484249-Adolescent,
pubmed-meshheading:8484249-Adult,
pubmed-meshheading:8484249-Aged,
pubmed-meshheading:8484249-Autoantibodies,
pubmed-meshheading:8484249-Blood Transfusion,
pubmed-meshheading:8484249-Child,
pubmed-meshheading:8484249-Child, Preschool,
pubmed-meshheading:8484249-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:8484249-Female,
pubmed-meshheading:8484249-Hematologic Diseases,
pubmed-meshheading:8484249-Humans,
pubmed-meshheading:8484249-Immunoblotting,
pubmed-meshheading:8484249-Infant,
pubmed-meshheading:8484249-Male,
pubmed-meshheading:8484249-Middle Aged,
pubmed-meshheading:8484249-Molecular Weight,
pubmed-meshheading:8484249-Platelet Membrane Glycoproteins,
pubmed-meshheading:8484249-Random Allocation
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Antibodies to platelet glycoprotein V in polytransfused patients with haematological disease.
|
| pubmed:affiliation |
South West Regional Transfusion Centre, Bristol, UK.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|