Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1993-5-28
|
| pubmed:abstractText |
We have studied the expression of IFN-gamma, IL-2 and IL-2 receptor (IL-2R) at the mRNA and protein levels in spleen and lymph node cells from Trypanosoma cruzi-infected BALB/c mice. At 21 days post infection (dpi) (peak of parasitaemia), spleen cells stimulated with Con A for 16 h showed a reduced IFN-gamma, IL-2 and IL-2R mRNA production compared with non-infected controls. Lymph node cells obtained at 4, 21 or 60 dpi produced similar amounts of IFN-gamma, IL-2 or IL-2R transcripts after mitogen stimulation as uninfected controls. Spleen cells obtained at 21 dpi showed a lower Con A proliferative response and IL-2R expression compared with non-infected controls, while the proliferation and IL-2R expression of lymph node cells at 21 dpi was unaltered. Supernatants from 48 h Con A-stimulated spleen and lymph node cells from mice at 21 dpi had very low levels of IL-2 but contained significantly higher levels of IFN-gamma compared with the supernatants of cells from non-infected mice. The latter phenomenon correlates with an accelerated rate of IFN-gamma mRNA accumulation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0300-9475
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
37
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
559-68
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8484102-Animals,
pubmed-meshheading:8484102-Blotting, Northern,
pubmed-meshheading:8484102-Cells, Cultured,
pubmed-meshheading:8484102-Chagas Disease,
pubmed-meshheading:8484102-Concanavalin A,
pubmed-meshheading:8484102-Flow Cytometry,
pubmed-meshheading:8484102-Gene Expression Regulation,
pubmed-meshheading:8484102-Interferon-gamma,
pubmed-meshheading:8484102-Interleukin-2,
pubmed-meshheading:8484102-Lymph Nodes,
pubmed-meshheading:8484102-Lymphocyte Activation,
pubmed-meshheading:8484102-Male,
pubmed-meshheading:8484102-Mice,
pubmed-meshheading:8484102-Mice, Inbred BALB C,
pubmed-meshheading:8484102-RNA, Messenger,
pubmed-meshheading:8484102-Receptors, Interleukin-2,
pubmed-meshheading:8484102-Spleen,
pubmed-meshheading:8484102-Time Factors,
pubmed-meshheading:8484102-Transcription, Genetic
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Organ-specific regulation of interferon-gamma, interleukin-2 and interleukin-2 receptor during murine infection with Trypanosoma cruzi.
|
| pubmed:affiliation |
Department of Immunology, Karolinska Institute, Stockholm, Sweden.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|