Linked Life Data

8482117

Source:http://linkedlifedata.com/resource/pubmed/id/8482117

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-6-1
pubmed:abstractText
Raman spectroscopy was used for the first time to characterize the structure of lipid membranes prepared from the nuclear and cortical regions of 48 and 69 year old clear human lenses. The interface region carbonyl band appears as a doublet at 1742 and 1728 cm-1. The lower frequency band is characteristic of a hydrogen bonded carbonyl group, perhaps to bilayer water. From the intensity of the curve fit bands, we calculate that 43% of the carbonyl groups are hydrogen bonded. Our data show that the hydrocarbon chains of the nuclear lipids are 1.4 times more saturated than those of the cortical lipids. The molar ratio of phospholipid CH2/= C-H groups was calculated to be 13 and 18 for cortical and nuclear lipids, respectively. Hydrocarbon chain disorder was estimated to be 72 and 58% (+/- 8% disorder) for the cortical and the nuclear lipids, respectively. Raman spectroscopy is sensitive to structural differences in various regions of the lipid bilayer and could be an effective tool to explore lipid and protein interactions in terms of lens region, age and opacity.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0271-3683
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
279-84
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Raman structural characterization of clear human lens lipid membranes.
pubmed:affiliation
Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Research Institute, University of Louisville School of Medicine 40292.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't