Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-5-21
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03139, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03140, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03141, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03142, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03143, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03144, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03145, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03146, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03147, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03148, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03149, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03150, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03151, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03152, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03153, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03154, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03155, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03156, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03157, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03158, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03159, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03160, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03161, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03162, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03163, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03164, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03165, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03166, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03167, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03168
pubmed:abstractText
A large number (33) of human Fab fragments reacting with HIV-1 surface glycoprotein gp120 have been generated by selection from a combinatorial IgG1 kappa library displayed on the surface of phage. The library was prepared from a long term asymptomatic HIV-seropositive donor. Analysis of the sequences from these Fabs shows the heavy chains can be placed in groups, many of which contain intraclonal variants, almost certainly corresponding to chains used in vivo. Further variants can be accessed via chain shuffling experiments in which a given light chain is recombined with a library of heavy chains. Heavy chain promiscuity, i.e. the ability of heavy chains to pair with different light chains with retention of antigen binding, is dependent on the particular heavy chain considered and probably excludes the identification of in vivo light chain partners. The antibodies examined here are primarily to the CD4 binding site on gp120 and broadly reflect the serum profile of the donor. The antibodies show evidence of extensive somatic modification indicative of an antigen-driven response. The heavy chain CDR3 regions of the antibodies show a remarkably conserved extended length. A number also show strong sequence conservation in CDR3 against a background of considerable diversity in the rest of the VH gene supporting a central role for this region in antigen recognition.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-2836
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
230
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
812-23
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Molecular profile of an antibody response to HIV-1 as probed by combinatorial libraries.
pubmed:affiliation
Department of Immunology, Scripps Research Institute, La Jolla, CA 92037.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't