Linked Life Data

8477667

Source:http://linkedlifedata.com/resource/pubmed/id/8477667

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-5-25
pubmed:abstractText
Although hCG and mouse epidermal growth factor (mEGF) activate different signaling systems in a clonal strain of murine Leydig tumor cells (designated MA-10), both compounds ultimately elicit several common effects, such as increased steroidogenesis, decreased transcription of the LH/CG receptor gene, and attenuation of adenylyl cyclase. Based on this information, it was hypothesized that hCG and mEGF may induce phosphorylation of a common protein or set of proteins. Studies were performed to identify such a protein if it exists. MA-10 cells were metabolically labeled with [32P]orthophosphate, and phosphorylation patterns were examined and quantitated using two-dimensional gel electrophoresis. A 21-kilodalton protein whose phosphorylation state increased after hCG and mEGF stimulation was identified. Three isoforms of this protein were visible after hCG stimulation, with approximate pI values of 5.9, 6.0, and 6.1, while only two isoforms were visible after mEGF stimulation, with approximate pI values of 6.0 and 6.1. This protein is phosphorylated on serine residues in response to either hCG or mEGF. Since this protein may play a role in eliciting the common actions of hCG and mEGF, its time course of phosphorylation and concentration responsiveness to hCG and mEGF were also characterized.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
132
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2229-38
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Identification of a Leydig tumor cell protein that is phosphorylated in response to stimulation with choriogonadotropin or epidermal growth factor.
pubmed:affiliation
Department of Pharmacology, University of Iowa College of Medicine, Iowa City 52242.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't