Linked Life Data

8476419

Source:http://linkedlifedata.com/resource/pubmed/id/8476419

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-5-20
pubmed:abstractText
The pTG7101 plasmid containing the full length human alpha 1-Antitrypsin was encapsulated in large (142 +/- 15 nm of diameter) and small (54 +/- 11 nm of diameter) liposomes and administered i.v. to mice (80 ng/mouse). Control animals were treated with empty (small and large) liposomes plus free DNA and with the liposome solvent buffer. The immunohistochemical results on liver cryosections and cytophotometric analysis of hepatocyte chromophore absorbance, after peroxidase reaction, indicated that significant presence of immunoreactive human alpha 1-antitrypsin was present 7 days after mice treatment with encapsulated DNA in small liposomes but not when large liposomes were used. This effect was observed in a great number of liver parenchymal cells. These results agree with the observation that only small liposomes have easy access to hepatocytes and support the idea that small liposomes are appropriate vehicles for in vivo delivery of specific genetic material to liver parenchymal cells, with high efficiency.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
192
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
174-81
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
In vivo delivery of human alpha 1-antitrypsin gene to mouse hepatocytes by liposomes.
pubmed:affiliation
Dpt. Pharmacology, Faculty of Medicine and Dentistry, University of Valencia, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't