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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4 Pt 1
|
| pubmed:dateCreated |
1993-5-17
|
| pubmed:abstractText |
We characterized the influence of phosphorylated sugars and cell surface sialic acids on the adherence of human polymorphonuclear leukocyte (PMN) to rat alveolar type II cell (ATII cells) and human-derived A549 cell monolayers in vitro. Percent adherence of radiolabeled polymorphonuclear leukocytes was assessed after incubating cells with the carbohydrates, enzymes, or lectins to be tested. Lactose-1-phosphate (Lact1P) and maltose-1-phosphate (Malt1P) (10 mM) inhibited adherence of PMN to ATII cells and A549 cells. Maximal inhibition followed treatment of both PMN and rat ATII cells and amounted to 85 +/- 7% with Lact1P and 92 +/- 3% with Malt1P. Inhibition was concentration dependent. Incubation of PMN with mannose-6-phosphate reduced adherence to rat ATII cells and A549 cells by 36 +/- 11 and 39 +/- 8%, respectively. Maximal concentrations of sugars did not alter cellular viability. Neuraminidase-induced desialilation of ATII cells increased adherence of PMN by 36 +/- 7% to rat ATII cells and by 86 +/- 18% to A549 cells. Masking of terminal sialic acids on rat ATH cells with Limulus polyphemus agglutinin (100 micrograms/ml) increased adherence by 50 +/- 2%. These results indicate that cell surface carbohydrates are involved in the regulation of the adhesive interaction between PMN and ATII cells in vitro.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0002-9513
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
264
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
L391-400
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8476068-Animals,
pubmed-meshheading:8476068-Cell Adhesion,
pubmed-meshheading:8476068-Cell Membrane,
pubmed-meshheading:8476068-Cells, Cultured,
pubmed-meshheading:8476068-Epithelial Cells,
pubmed-meshheading:8476068-Epithelium,
pubmed-meshheading:8476068-Humans,
pubmed-meshheading:8476068-Kinetics,
pubmed-meshheading:8476068-Lung Neoplasms,
pubmed-meshheading:8476068-Male,
pubmed-meshheading:8476068-Mannosephosphates,
pubmed-meshheading:8476068-Microscopy, Electron,
pubmed-meshheading:8476068-Neutrophils,
pubmed-meshheading:8476068-Pulmonary Alveoli,
pubmed-meshheading:8476068-Rats,
pubmed-meshheading:8476068-Rats, Sprague-Dawley,
pubmed-meshheading:8476068-Sugar Phosphates,
pubmed-meshheading:8476068-Tumor Cells, Cultured
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Cell surface carbohydrates modulate neutrophil adherence to alveolar type II cells in vitro.
|
| pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale U. 226, Paris, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|