Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-5-18
pubmed:databankReference
pubmed:abstractText
Ribosomal protein L1 from Saccharomyces cerevisiae binds 5S rRNA and can be released from intact 60S ribosomal subunits as an L1-5S ribonucleoprotein (RNP) particle. To understand the nature of the interaction between L1 and 5S rRNA and to assess the role of L1 in ribosome assembly and function, we cloned the RPL1 gene encoding L1. We have shown that RPL1 is an essential single-copy gene. A conditional null mutant in which the only copy of RPL1 is under control of the repressible GAL1 promoter was constructed. Depletion of L1 causes instability of newly synthesized 5S rRNA in vivo. Cells depleted of L1 no longer assemble 60S ribosomal subunits, indicating that L1 is required for assembly of stable 60S ribosomal subunits but not 40S ribosomal subunits. An L1-5S RNP particle not associated with ribosomal particles was detected by coimmunoprecipitation of L1 and 5S rRNA. This pool of L1-5S RNP remained stable even upon cessation of 60S ribosomal subunit assembly by depletion of another ribosomal protein, L16. Preliminary results suggest that transcription of RPL1 is not autogenously regulated by L1.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-152199, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-1545793, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-1557378, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-1628625, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-1716386, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-1737784, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-1763709, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-1825809, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-1922070, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-1996092, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-2007570, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-2040015, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-2137374, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-2198535, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-2277060, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-2406561, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-2476649, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-2477156, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-2586520, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-2642897, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-2649882, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-2659436, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-2666845, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-284338, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3018530, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3047007, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3052292, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3063604, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3118184, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-321249, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3275866, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3279045, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3282992, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3308884, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3323813, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3329033, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3537704, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3624282, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-3897837, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-393511, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-4557192, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-4586940, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-5001943, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-5540996, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-6092912, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-6204768, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-6206783, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-6248420, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-6310324, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-6312838, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-6336730, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-6421579, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-6765595, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-7001457, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-7408887, http://linkedlifedata.com/resource/pubmed/commentcorrection/8474444-781671
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2835-45
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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