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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1993-5-14
|
| pubmed:databankReference | |
| pubmed:abstractText |
Five members of the human growth hormone (GH) gene family are located at a single locus on chromosome 17. Growth hormone is expressed in the pituitary under the control of the tissue-specific factor Pit 1/GHF-1, and chorionic somatomammotropin (CS) -A, -B, and -L, as well as placental GH variant, are expressed specifically in the placental syncytiotrophoblast. Despite this specificity in vivo, the CS-A promoter can bind Pit 1/GHF-1 and allow CS-A promoter activity in pituitary tumor cells after gene transfer. We have identified and characterized PSF sequences associated with only the placental members in the GH/CS locus which repress placental promoter activity > 90% in transfected pituitary cells. These sequences do not significantly affect promoter function in placental cells after gene transfer. Repressor activity correlates with binding of protein at two sites (PSF-A and PSF-B) with pituitary, but not placental, nuclear extracts. Competition studies suggest an interaction between PSF and Pit 1/GHF-1 proteins. These results indicate that PSF protein can repress CS-A promoter activity in a tissue-specific manner in vitro and provide a possible mechanism by which expression of placental members of the GH family are inhibited in the pituitary in vivo.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/GH2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Placental Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
268
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
8473-9
|
| pubmed:dateRevised |
2010-9-21
|
| pubmed:meshHeading |
pubmed-meshheading:8473291-Animals,
pubmed-meshheading:8473291-Base Sequence,
pubmed-meshheading:8473291-Binding Sites,
pubmed-meshheading:8473291-DNA,
pubmed-meshheading:8473291-Gene Expression Regulation,
pubmed-meshheading:8473291-Growth Hormone,
pubmed-meshheading:8473291-Humans,
pubmed-meshheading:8473291-Molecular Sequence Data,
pubmed-meshheading:8473291-Multigene Family,
pubmed-meshheading:8473291-Oligonucleotides,
pubmed-meshheading:8473291-Organ Specificity,
pubmed-meshheading:8473291-Pituitary Gland,
pubmed-meshheading:8473291-Pituitary Neoplasms,
pubmed-meshheading:8473291-Placenta,
pubmed-meshheading:8473291-Placental Hormones,
pubmed-meshheading:8473291-Rats,
pubmed-meshheading:8473291-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8473291-Repressor Proteins,
pubmed-meshheading:8473291-Tumor Cells, Cultured
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Pituitary-specific repression of placental members of the human growth hormone gene family. A possible mechanism for locus regulation.
|
| pubmed:affiliation |
Department of Physiology, University of Manitoba, Winnipeg, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|