Linked Life Data

8470806

Source:http://linkedlifedata.com/resource/pubmed/id/8470806

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-5-11
pubmed:abstractText
Akabayashi et al. made a valuable modification of the enzymatic methods from our laboratory for measuring 2-deoxyglucose and 2-deoxyglucose 6-phosphate. Their modified procedure eliminates glucose and glucose 6-phosphate by conversion to fructose-1,6-bisphosphate, thereby saving two analytical steps. However, the present report describes a limitation of this new elimination procedure which is due to its unexpected reversibility, and provides an easy way to circumvent this limitation, namely heating to destroy the reagent enzymes before proceeding. The final result is a more flexible analytical scheme that is capable of measuring 2-deoxyglucose and its phosphate down to extremely low levels in the presence of up to thousandfold higher glucose concentrations. The completeness of glucose elimination eliminates both the problem of contamination of available glucose-6-phosphate dehydrogenases with 6-phosphogluconate dehydrogenase, and also the effect of the presence in this same enzyme of a trace of glucose dehydrogenase activity, which is an apparent side reaction.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:volume
209
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
335-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
A refinement of the Akabayashi-Saito-Kato modification of the enzymatic methods for 2-deoxyglucose and 2-deoxyglucose 6-phosphate.
pubmed:affiliation
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.