Linked Life Data

8467645

Source:http://linkedlifedata.com/resource/pubmed/id/8467645

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-5-13
pubmed:abstractText
The current methods for measurement of free cortisol are hampered by methodological drawbacks including large sample requirement, length of the procedure, and overestimation or underestimation of free cortisol. We report a technique combining ultrafiltration and dialysis, which could largely overcome these drawbacks, for measurement of the per cent free cortisol in plasma in the dog. This is a modification of the method of Hammond et al. for the determination of sex steroids in man. We found a good positive correlation (rs = 0.87) with equilibrium dialysis, which is considered the golden standard in free cortisol measurements. The 2.5th and 97.5th percentiles of values of free cortisol measured in plasma of 40 healthy household dogs were 5-20 nmol/l or 5.1-11.7% of the total cortisol concentration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0739-7240
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-53
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Determination of the percentage of free cortisol in plasma in the dog by ultrafiltration/dialysis.
pubmed:affiliation
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
pubmed:publicationType
Journal Article