Linked Life Data

8466960

Source:http://linkedlifedata.com/resource/pubmed/id/8466960

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-5-13
pubmed:abstractText
Antioxidants are added to foods and drugs to inhibit their oxidation. As these additives are somewhat toxic, it is necessary to control the amount added to any food or drug. Liquid chromatography (LC) is a powerful tool for this purpose. Many antioxidants are electroactive molecules which enables the advantages of electrochemical detection or selectivity and sensitivity to be realized. The interest of analysts in microbore LC arises from the low mobile phase volumetric flow rates involved, the reduced on-column samples together with reduced chromatographic dilution and high efficiency. Coupling of microbore LC with electrochemical detection adds another advantage: the decrease of electrode ageing. The problem of extra-column band broadening with microbore column is discussed in the present communication. A micro LC-electrochemical detection system is constructed and tested using catecholamines. The limit of detection (LOD) for noradrenaline using a 0.7 mm bore column is found to be 0.1 pg injected in 0.2 microliter (0.6 femtomoles). Three phenolic antioxidants are studied: tert-butyl-p-hydroxyanisole (BHA), di-tert-butyl-hydroxytoluene (BHT) and n-propyl gallate (3,4,5-trimethoxybenzoic acid propyl ester). The dynamic range is four orders of magnitude with LODs down to 0.1 femtomoles (20 fg injected) with a 0.3 mm bore column. No electrode response change is observed after 60 injections of 3 ng BHA over 6 days. Antioxidants are determined in different pharmaceutical preparations and foodstuffs (chewing gums, dried potato flakes). The agreement between the manufacturer stated concentrations and observed results is found to be satisfactory.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
71-8
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Microbore liquid chromatography with electrochemical detection for the control of phenolic antioxidants in drugs and foods.
pubmed:affiliation
Laboratoire des Sciences Analytiques, UA CNRS 435, Université de Lyon 1, Villeurbanne, France.
pubmed:publicationType
Journal Article