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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6419
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pubmed:dateCreated |
1993-4-30
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pubmed:abstractText |
The transcription factor GATA-1 is a fundamental regulator of genes in haematopoietic cell lineages and belongs to a family of factors that bind to the consensus sequence WGATAR. The GATA motif was originally identified in cis-regulatory regions of globin and other erythroid-specific genes, but the range of genes controlled by the GATA factors has since expanded. Members of the GATA transcription factor family share a conserved zinc-finger DNA-binding domain, but the expression profile of each GATA factor is distinct. Here we show that a testis form of murine (m)GATA-1 messenger RNA is transcribed from a promoter located 5' to the erythroid first exon, and the remaining exons (which encode the mGATA-1 protein) are used in common by both testis and erythroid transcripts. We use an anti-mGATA-1 monoclonal antibody to show that the factor expressed in erythroid cells is the same as that found in the seminiferous tubules of murine testis. The GATA-1-expressing cells in 10-week-old testis were found only in contact with the basement membrane of seminiferous tubules, suggesting that GATA-1 regulates genes during the earliest stages of spermatogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
362
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
466-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8464479-Aging,
pubmed-meshheading:8464479-Animals,
pubmed-meshheading:8464479-Base Sequence,
pubmed-meshheading:8464479-DNA-Binding Proteins,
pubmed-meshheading:8464479-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:8464479-GATA1 Transcription Factor,
pubmed-meshheading:8464479-Gene Expression,
pubmed-meshheading:8464479-Gene Library,
pubmed-meshheading:8464479-Male,
pubmed-meshheading:8464479-Mice,
pubmed-meshheading:8464479-Molecular Sequence Data,
pubmed-meshheading:8464479-Oligodeoxyribonucleotides,
pubmed-meshheading:8464479-Organ Specificity,
pubmed-meshheading:8464479-Polymerase Chain Reaction,
pubmed-meshheading:8464479-RNA, Messenger,
pubmed-meshheading:8464479-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8464479-Testis,
pubmed-meshheading:8464479-Transcription, Genetic,
pubmed-meshheading:8464479-Transcription Factors,
pubmed-meshheading:8464479-Zinc Fingers
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pubmed:year |
1993
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pubmed:articleTitle |
Erythroid transcription factor GATA-1 is abundantly transcribed in mouse testis.
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pubmed:affiliation |
Department of Pediatrics, Hirosaki University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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