Linked Life Data

8464383

Source:http://linkedlifedata.com/resource/pubmed/id/8464383

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-5-6
pubmed:abstractText
The Chinese hamster irs2 cell line shows cellular hypersensitivity to ionizing radiation although the induction and repair of double-strand breaks (dsb) in bulk DNA is normal. Here we report that irs2 shows chromosomal hypersensitivity to ionizing radiation and the restriction endonuclease PvuII. The ratio of induced chromosomal aberrations in irs2 versus V79 was similar to that for survival (factor of between 2 and 4). PvuII was administered during cell poration with the bacterial toxin streptolysin O. We also report that when streptolysin O porated irs2 and V79 cells were treated with PvuII, and dsb assayed by neutral filter elution, equivalent numbers of dsb were induced in the two lines as a function of time following treatment. Our data show that irs2 has a DNA damage processing defect that leads to enhanced conversion of blunt-ended dsb into visible chromosomal damage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0267-8357
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
141-7
pubmed:dateRevised
2008-8-19
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Radiosensitive Chinese hamster irs2 cells show enhanced chromosomal sensitivity to ionizing radiation and restriction endonuclease induced blunt-ended double-strand breaks.
pubmed:affiliation
School of Biological and Medical Sciences, University of St Andrews, Fife, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't