|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1993-4-30
|
|
pubmed:abstractText |
A 50-kDa protein has been purified to homogeneity from free mRNP of rabbit reticulocytes. This protein, designated as p50, is present within both free mRNP (approximately 4 mol protein/mol globin mRNA) and polyribosomal mRNP (approximately 2 mol protein/mol globin mRNA). p50 is a basic protein (pI approximately 9.5) and is characterized by a high glycine content of approximately 20%. Nitrocellulose-filter analysis has shown that p50 interacts with globin mRNA with an association constant of approximately 2.5 x 10(8) M-1 (100 mM KAc, 4 degrees C). Various RNA and polyribonucleotides have the following relative affinity for p50; poly(G) > poly(U) > globin mRNA approximately 16S rRNA > poly(A) > poly(C). p50 can be phosphorylated both in vitro and in vivo.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0014-2956
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
212
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
633-8
|
|
pubmed:dateRevised |
2007-7-23
|
|
pubmed:meshHeading |
pubmed-meshheading:8462540-Amino Acids,
pubmed-meshheading:8462540-Animals,
pubmed-meshheading:8462540-Cell Fractionation,
pubmed-meshheading:8462540-Cytoplasm,
pubmed-meshheading:8462540-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8462540-Globins,
pubmed-meshheading:8462540-Molecular Weight,
pubmed-meshheading:8462540-Phosphorylation,
pubmed-meshheading:8462540-Polyribosomes,
pubmed-meshheading:8462540-RNA, Messenger,
pubmed-meshheading:8462540-Rabbits,
pubmed-meshheading:8462540-Repressor Proteins,
pubmed-meshheading:8462540-Reticulocytes,
pubmed-meshheading:8462540-Ribonucleoproteins,
pubmed-meshheading:8462540-Ribosomes
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
Purification and characterization of the major 50-kDa repressor protein from cytoplasmic mRNP of rabbit reticulocytes.
|
|
pubmed:affiliation |
Institute of Protein Research, Russian Academy of Sciences, Pushchino.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
