rdf:type |
|
lifeskim:mentions |
umls-concept:C0007586,
umls-concept:C0009491,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0031715,
umls-concept:C0033684,
umls-concept:C0037993,
umls-concept:C0205314,
umls-concept:C0220781,
umls-concept:C0585064,
umls-concept:C0597304,
umls-concept:C0679622,
umls-concept:C1148673,
umls-concept:C1521970,
umls-concept:C1883254
|
pubmed:issue |
2
|
pubmed:dateCreated |
1993-5-3
|
pubmed:abstractText |
1. Cultured mouse spleen cells were exposed to the mitogen Concanavalin A followed by isoproterenol, and nuclei were electronically sorted from seven partitions of the cell cycle. 2. Several nuclear proteins, including stress proteins, which were cell-cycle-stage specific, were elicited by isoproterenol as determined by micro-electrophoresis and fluorography. 3. Two novel S-phase proteins (X0 and X') demonstrated differing synthesis and phosphorylation patterns during the cell-cycle phases. 4. X' showed DNA binding characteristics and proteolytic properties (hydrolyzing X0 or beta-galactosidase); both proteins were cell-cycle regulated.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0305-0491
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
104
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
361-70
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:8462285-Animals,
pubmed-meshheading:8462285-Cell Cycle,
pubmed-meshheading:8462285-Cells, Cultured,
pubmed-meshheading:8462285-Concanavalin A,
pubmed-meshheading:8462285-DNA,
pubmed-meshheading:8462285-Hydrolysis,
pubmed-meshheading:8462285-Isoproterenol,
pubmed-meshheading:8462285-Male,
pubmed-meshheading:8462285-Methionine,
pubmed-meshheading:8462285-Mice,
pubmed-meshheading:8462285-Mice, Inbred C57BL,
pubmed-meshheading:8462285-Nuclear Proteins,
pubmed-meshheading:8462285-Phenylmethylsulfonyl Fluoride,
pubmed-meshheading:8462285-Phosphorylation,
pubmed-meshheading:8462285-Protein Binding,
pubmed-meshheading:8462285-Spleen
|
pubmed:year |
1993
|
pubmed:articleTitle |
Comparative studies of synthesis, phosphorylation, DNA binding and proteolytic characteristics of a novel protein during phases of the mouse spleen cell cycle.
|
pubmed:affiliation |
Department of Health and Human Services, Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR 72079.
|
pubmed:publicationType |
Journal Article,
Comparative Study
|