Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1993-4-23
|
| pubmed:abstractText |
Mutation sites of unique temperature-sensitive (ts) mutants of Abelson murine leukemia virus(A-MuLV) that exhibited the ts phenotype in colony-forming ability in soft agar, but not in morphological transformation, were determined. Cloning and sequencing analysis of the full viral genomes of five independent ts mutants revealed a total of 10 mutation sites: 3 mutations (bp714, 742, and 817) located in the 5' untranslated region between LTR and gag gene; 4 mutations (bp1227, 1229, 1512, and 1634) in the gag gene; 3 mutations (point mutations at bp2764 and 3265, and 2-base deletion of bp3448 and 3449) in the abl gene. To determine the mutation sites critical for the ts phenotype in colony-forming ability in soft agar, hybrid viruses were constructed by exchanging the corresponding restriction fragments between wild-type and ts A-MuLV. The hybrid virus containing only the point mutation (T-->G) at bp2764 that exchanged leucine with arginine exhibited the ts phenotype in colony-forming ability in soft agar. Thus, a novel mutation within the kinase domain of the abl gene critical for the ts phenotype in colony-forming ability in soft agar was determined here.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0042-6822
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
193
|
| pubmed:geneSymbol |
gag,
v-abl
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
967-70
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:8460498-3T3 Cells,
pubmed-meshheading:8460498-Abelson murine leukemia virus,
pubmed-meshheading:8460498-Agar,
pubmed-meshheading:8460498-Amino Acid Sequence,
pubmed-meshheading:8460498-Animals,
pubmed-meshheading:8460498-Base Sequence,
pubmed-meshheading:8460498-Cell Line,
pubmed-meshheading:8460498-Cloning, Molecular,
pubmed-meshheading:8460498-Genes, abl,
pubmed-meshheading:8460498-Genes, gag,
pubmed-meshheading:8460498-Genome, Viral,
pubmed-meshheading:8460498-Mice,
pubmed-meshheading:8460498-Mutation,
pubmed-meshheading:8460498-Oncogene Proteins v-abl,
pubmed-meshheading:8460498-Phenotype,
pubmed-meshheading:8460498-Point Mutation,
pubmed-meshheading:8460498-Protein-Tyrosine Kinases,
pubmed-meshheading:8460498-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:8460498-Sequence Deletion,
pubmed-meshheading:8460498-Temperature
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
A novel mutation within the kinase domain of v-abl gene responsible for temperature-sensitive colony-forming ability in soft agar.
|
| pubmed:affiliation |
Osaka University Medical School, Department of Medicine III, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|