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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1993-4-29
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pubmed:databankReference |
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pubmed:abstractText |
Mycobacteriophage L5 is a temperate phage of the mycobacteria that forms stable lysogens in Mycobacterium smegmatis. We show here that the 183-amino-acid product of L5 gene 71 confers immunity to L5 superinfection, is required for maintenance of the lysogenic state and contains a helix-turn-helix DNA-binding motif--properties associated with repressors of temperate phages. We have utilized these observations to demonstrate the use of L5 gene 71 as a selectable marker for genetic transformation of the mycobacteria. Significantly, the use of L5 gene 71 as a selectable gene avoids the requirement for antibiotic-resistance genes providing an important tool for manipulation of the pathogens Mycobacterium tuberculosis and Mycobacterium avium, and for the construction of recombinant BCG vaccines.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0950-382X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
407-17
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8459767-Amino Acid Sequence,
pubmed-meshheading:8459767-Base Sequence,
pubmed-meshheading:8459767-DNA, Bacterial,
pubmed-meshheading:8459767-DNA, Recombinant,
pubmed-meshheading:8459767-DNA Mutational Analysis,
pubmed-meshheading:8459767-DNA-Binding Proteins,
pubmed-meshheading:8459767-Genes, Viral,
pubmed-meshheading:8459767-Genetic Markers,
pubmed-meshheading:8459767-Hot Temperature,
pubmed-meshheading:8459767-Lysogeny,
pubmed-meshheading:8459767-Molecular Sequence Data,
pubmed-meshheading:8459767-Mycobacteriaceae,
pubmed-meshheading:8459767-Mycobacteriophages,
pubmed-meshheading:8459767-Phenotype,
pubmed-meshheading:8459767-Plasmids,
pubmed-meshheading:8459767-Restriction Mapping,
pubmed-meshheading:8459767-Transformation, Genetic,
pubmed-meshheading:8459767-Viral Plaque Assay,
pubmed-meshheading:8459767-Viral Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
Superinfection immunity of mycobacteriophage L5: applications for genetic transformation of mycobacteria.
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pubmed:affiliation |
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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