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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1993-4-22
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pubmed:abstractText |
Systemic and mucosal B-cell-mediated immune responses to purified filamentous hemagglutinin (FHA) in mice were analyzed at different times following a single respiratory infection with Bordetella pertussis. Serum immunoglobulin G (IgG) anti-FHA and respiratory IgG and IgA anti-FHA antibodies were first detected at 3 weeks postinfection, reached high levels by 8 weeks postinfection, and remained at high levels 12 to 32 weeks postinfection. FHA-specific B lymphocytes isolated from the spleens or lungs of uninfected control mice or mice convalescing from B. pertussis respiratory infection were analyzed in limiting-dilution cultures. Analysis of culture supernatants for the production of antibodies to FHA revealed an increased frequency of FHA-specific B cells of both the IgG- and the IgA-secreting classes in the lungs and tracheas of aerosol-challenged mice; these levels remained high as late as 25 weeks postinfection, compared with those in uninfected controls. No corresponding increase in the frequency of FHA-specific B cells in the spleens of aerosol-infected mice was observed. This long-lasting response observed in cultured cells was radiation resistant, a result suggesting that this response was due to B cells already activated in vivo. Polymerase chain reaction analysis revealed low but detectable levels of B. pertussis chromosomal DNA in 75% of mice tested at 8 weeks postinfection and 37.5% of mice tested at 26 weeks postinfection, at which times high levels of anti-FHA antibody were detected. One explanation for these data may be that, in this animal model, a major adhesin of B. pertussis can persist and interact with components of the immune system to stimulate the production of specific antibody in the respiratory tract many weeks after a single B. pertussis infection.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-14279983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-1548072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-1562663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-1658537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-1696934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-1919361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-1937833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-2085491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-2294058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-2295882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-2313126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-2322466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-2364431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-2484300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-2547718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-2872102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-3257233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-3263573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-3874828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-4071052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-4176640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-4318529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-4318530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-4543318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-6249758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-6537815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8454349-6886900
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1447-52
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8454349-Animals,
pubmed-meshheading:8454349-Antibodies, Bacterial,
pubmed-meshheading:8454349-Antigens, Bacterial,
pubmed-meshheading:8454349-B-Lymphocytes,
pubmed-meshheading:8454349-Bacterial Adhesion,
pubmed-meshheading:8454349-Bacterial Proteins,
pubmed-meshheading:8454349-Bordetella pertussis,
pubmed-meshheading:8454349-Female,
pubmed-meshheading:8454349-Hemagglutinins,
pubmed-meshheading:8454349-Lung,
pubmed-meshheading:8454349-Mice,
pubmed-meshheading:8454349-Respiratory System,
pubmed-meshheading:8454349-Spleen,
pubmed-meshheading:8454349-Time Factors,
pubmed-meshheading:8454349-Whooping Cough
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pubmed:year |
1993
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pubmed:articleTitle |
Long-lived respiratory immune response to filamentous hemagglutinin following Bordetella pertussis infection.
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pubmed:affiliation |
Laboratory of Pertussis, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892-0029.
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pubmed:publicationType |
Journal Article
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