Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-4-22
pubmed:abstractText
Cytochrome P450cam (P450CIA1) catalyzes the hydroxylation of camphor and several substrate analogues such as norcamphor and 1-methyl-norcamphor. Hydroxylation was found experimentally at the 3, 5, and 6 positions of norcamphor, but only at the 5 and 6 positions of 1-methyl-norcamphor. In the catalytic cycle, the hydroxylation of substrate is coupled to the consumption of NADH. For camphor, the degree of coupling is 100%, but for both norcamphor and 1-methyl-norcamphor, the efficiency is dramatically lowered to 12% and 50%, respectively. Based on an examination of the active site of P450cam, it appeared that mutating position 185 might dramatically alter the product specificity and coupling of hydroxylation of norcamphor by P450cam. Analysis of molecular dynamics trajectories of norcamphor bound to the T185F mutant of cytochrome P450cam predicted that hydroxylation at the 3 position should be abolished and that the coupling should be dramatically increased. This mutant was constructed and the product profile and coupling experimentally determined. The coupling was doubled, and hydroxylation at the 3 position was essentially abolished. Both of these results are in agreement with the prediction.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1100841, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1326281, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1372979, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1449498, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1474394, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1537455, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1594575, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1631041, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1667879, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1741957, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1749772, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1784217, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1856184, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-1993199, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-2613690, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-2713354, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-2738924, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-2992468, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-3003058, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-3054871, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-3198602, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-3343243, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-364937, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-3656428, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-4085416, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-6696444, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-672627, http://linkedlifedata.com/resource/pubmed/commentcorrection/8453374-875032
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0961-8368
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
357-65
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Controlling the regiospecificity and coupling of cytochrome P450cam: T185F mutant increases coupling and abolishes 3-hydroxynorcamphor product.
pubmed:affiliation
Molecular Science Research Center, Pacific Northwest Laboratory, Richland, Washington 99352.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't