rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1993-4-9
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pubmed:abstractText |
We described here an approach for mapping proteins on any sequence of genomic DNA. UV-induced DNA-protein crosslinking within whole cells and the 'protein image' hybridization technique (1) were applied to test the proteins bound to different regions of the D. melanogaster hsp-70 gene. The histone H1-DNA association with the coding region is shown to be maintained, even during very intensive transcription, but is absent in the promoter. Two non-histone proteins with apparent molecular masses of 50 kD (p50) and 100 kD (p100) are crosslinked only to the active hsp-70 gene regulatory region and preferentially bind to its complementary and coding DNA strands, respectively.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-110462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-117907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-1310672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-1436067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2017359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2029271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2077684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2098005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2502314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2602113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2770546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2803304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2993917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2996773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-3018544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-3100052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-3685975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6280035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6325944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6326095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6430695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6436689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6472466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6540147
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Feb
|
pubmed:issn |
0305-1048
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:day |
25
|
pubmed:volume |
21
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pubmed:geneSymbol |
hsp-70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1031-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8451170-Animals,
pubmed-meshheading:8451170-Binding Sites,
pubmed-meshheading:8451170-Cells, Cultured,
pubmed-meshheading:8451170-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:8451170-DNA-Binding Proteins,
pubmed-meshheading:8451170-Drosophila melanogaster,
pubmed-meshheading:8451170-Heat-Shock Proteins,
pubmed-meshheading:8451170-Histones,
pubmed-meshheading:8451170-Promoter Regions, Genetic,
pubmed-meshheading:8451170-Transcription, Genetic,
pubmed-meshheading:8451170-Ultraviolet Rays
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pubmed:year |
1993
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pubmed:articleTitle |
Two non-histone proteins are associated with the promoter region and histone HI with the transcribed region of active hsp-70 genes as revealed by UV-induced DNA-protein crosslinking in vivo.
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pubmed:affiliation |
W.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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