Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-4-9
pubmed:abstractText
We described here an approach for mapping proteins on any sequence of genomic DNA. UV-induced DNA-protein crosslinking within whole cells and the 'protein image' hybridization technique (1) were applied to test the proteins bound to different regions of the D. melanogaster hsp-70 gene. The histone H1-DNA association with the coding region is shown to be maintained, even during very intensive transcription, but is absent in the promoter. Two non-histone proteins with apparent molecular masses of 50 kD (p50) and 100 kD (p100) are crosslinked only to the active hsp-70 gene regulatory region and preferentially bind to its complementary and coding DNA strands, respectively.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-110462, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-117907, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-1310672, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-1436067, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2017359, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2029271, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2077684, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2098005, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2502314, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2602113, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2770546, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2803304, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2993917, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-2996773, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-3018544, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-3100052, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-3685975, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6280035, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6325944, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6326095, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6430695, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6436689, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6472466, http://linkedlifedata.com/resource/pubmed/commentcorrection/8451170-6540147
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
21
pubmed:geneSymbol
hsp-70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1031-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Two non-histone proteins are associated with the promoter region and histone HI with the transcribed region of active hsp-70 genes as revealed by UV-induced DNA-protein crosslinking in vivo.
pubmed:affiliation
W.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't