Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-4-9
pubmed:abstractText
By immunoprecipitation analysis, enhanced p53 expression was detected in 3 of 4 adult T-cell leukemia (ATL) cell lines, 1 of 3 HTLV-I-infected cell lines and 1 of 5 fresh ATL samples, compared with phytohemagglutinin-stimulated peripheral blood lymphocytes. Among these 5 high expressers, p53 missense mutations were indicated in 2 ATL cell lines and 1 fresh ATL sample by extensive p53 cDNA and genomic DNA polymerase chain reaction single-strand conformation polymorphism analysis. No mutation was found throughout the entire coding region of the remaining 2 high expressers (1 ATL and 1 HTLV-I-infected cell lines) and low expressers of p53 (2 HTLV-I-infected cell lines). Tax oncoprotein expression was found in these 2 high p53 expressers in which p53 mutation was not present, but not in low p53 expressers or cells carrying this mutation. The levels of p53 mRNA were similar among the samples regardless of p53 levels. Posttranscriptional mechanisms other than missense mutation would thus appear to increase p53 in the Tax-expressing cells but not in cells containing undetectable levels of Tax. No complex formation between p53 and Tax was observed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0910-5050
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:geneSymbol
p53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:8449826-Adult, pubmed-meshheading:8449826-Base Sequence, pubmed-meshheading:8449826-Cell Line, pubmed-meshheading:8449826-Gene Expression, pubmed-meshheading:8449826-Gene Products, tax, pubmed-meshheading:8449826-Genes, p53, pubmed-meshheading:8449826-Genes, pX, pubmed-meshheading:8449826-HTLV-I Infections, pubmed-meshheading:8449826-Human T-lymphotropic virus 1, pubmed-meshheading:8449826-Humans, pubmed-meshheading:8449826-Immunoblotting, pubmed-meshheading:8449826-Leukemia-Lymphoma, Adult T-Cell, pubmed-meshheading:8449826-Molecular Sequence Data, pubmed-meshheading:8449826-Oligodeoxyribonucleotides, pubmed-meshheading:8449826-Polymerase Chain Reaction, pubmed-meshheading:8449826-Restriction Mapping, pubmed-meshheading:8449826-Tumor Cells, Cultured, pubmed-meshheading:8449826-Tumor Suppressor Protein p53
pubmed:year
1993
pubmed:articleTitle
Aberrant expression of the p53 tumor suppressor gene in adult T-cell leukemia and HTLV-I-infected cells.
pubmed:affiliation
Department of Medicine, Kochi Medical School.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't