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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-4-6
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pubmed:abstractText |
Proteins of 14,000 daltons (SLP-14) were isolated and partially characterized from mouse mammary glands of different developmental stages. The purified proteins were partially sequenced at the amino acid level. The SLP-14 belong to the family of fatty acid-binding (FABP) proteins. The major SLP-14 expressed was a reflection of the differentiation stage of the mammary gland. Within the regions sequenced for each protein, virgin mouse mammary gland (primarily adipocytes) expressed a protein 100% homologous to adipocyte lipid binding protein whereas pregnant and lactating mouse mammary glands expressed a protein highly homologous to mouse heart fatty acid binding protein. In the lactating gland, the isolated protein was 97% identical to the heart FABP over a 98 amino acid stretch. We could not detect the 14Kd protein identified as mammary gland growth inhibitor (MDGI) which is also a member of the FABP family and highly homologous to heart FABP. A rabbit antiserum to the rat mammary SLP-14 recognized the SLP-14 proteins in mouse and rat mammary gland, skeletal muscle and heart, whereas it failed to recognize the SLP-14 in liver, intestine and other organs. These data indicate that the SLP-14 detected in rodent mammary gland is of the fatty acid-binding protein family. On the basis of amino acid sequence, the major form in the differentiated mouse mammary gland is apparently FABP and not MDGI. MDGI may be a protein in low abundance and/or localized to a specific group of mammary epithelial cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fabp5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Fabp7 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Fabp7 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acid-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Selenium
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
191
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
61-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8447836-Amino Acid Sequence,
pubmed-meshheading:8447836-Animals,
pubmed-meshheading:8447836-Antibodies,
pubmed-meshheading:8447836-Carrier Proteins,
pubmed-meshheading:8447836-Chromatography, Gel,
pubmed-meshheading:8447836-Chromatography, Ion Exchange,
pubmed-meshheading:8447836-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:8447836-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8447836-Fatty Acid-Binding Proteins,
pubmed-meshheading:8447836-Fatty Acids,
pubmed-meshheading:8447836-Female,
pubmed-meshheading:8447836-Lactation,
pubmed-meshheading:8447836-Mammary Glands, Animal,
pubmed-meshheading:8447836-Mice,
pubmed-meshheading:8447836-Mice, Inbred BALB C,
pubmed-meshheading:8447836-Molecular Sequence Data,
pubmed-meshheading:8447836-Molecular Weight,
pubmed-meshheading:8447836-Myocardium,
pubmed-meshheading:8447836-Neoplasm Proteins,
pubmed-meshheading:8447836-Nerve Tissue Proteins,
pubmed-meshheading:8447836-Organ Specificity,
pubmed-meshheading:8447836-Pregnancy,
pubmed-meshheading:8447836-Pregnancy, Animal,
pubmed-meshheading:8447836-Selenium,
pubmed-meshheading:8447836-Sequence Homology, Amino Acid
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pubmed:year |
1993
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pubmed:articleTitle |
Expression of fatty acid-binding proteins in the developing mouse mammary gland.
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pubmed:affiliation |
Dept. of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
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pubmed:publicationType |
Journal Article,
Comparative Study
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