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rdf:type |
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lifeskim:mentions |
umls-concept:C0004266,
umls-concept:C0007634,
umls-concept:C0021585,
umls-concept:C0025246,
umls-concept:C0332120,
umls-concept:C0439851,
umls-concept:C0935640,
umls-concept:C1550548,
umls-concept:C1552596,
umls-concept:C1555714,
umls-concept:C1705654,
umls-concept:C1947931
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pubmed:issue |
4
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pubmed:dateCreated |
1993-4-8
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pubmed:abstractText |
This research provides the first evidence for specific receptor binding of polyhedron-derived baculovirus (PDV) to host cells and to lepidopteran brush border membrane vesicles (BBMV) and demonstration of entry via a nonendocytotic pathway involving direct membrane fusion. The technique of fluorescence-activated cell sorting analysis was used to investigate the specificity of binding between the PDV phenotype of Lymantria dispar nuclear polyhedrosis virus (LdNPV) and host membranes. Fluorescein isothiocyanate-labeled PDV was found to bind in a saturable manner to the gypsy moth cell line IPLB-LdEIta and to L. dispar BBMV. The IPLB-LdEIta cell line was found to possess approximately 10(6) PDV-specific receptor sites per cell. Excess levels of unlabeled PDV were highly efficient in competing with fluorescein isothiocyanate-labeled PDV for limited receptor sites, further supporting the specificity of the interaction. Major reductions in virus binding (as high as 70%) after protease treatment of cells indicated that a protein receptor is involved. A fluorescence dequenching assay of membrane fusion with octadecyl rhodamine B (R18)-labeled PDV was used to identify PDV fusion to host cells and BBMV. Direct membrane fusion of PDV occurred at 27 degrees C to both target membranes as well as at 4 degrees C at approximately 55% of the levels achieved at 27 degrees C. Viral fusion to BBMV occurred throughout the pH range of 4 to 11, with dramatically increased fusion levels (threefold) under the alkaline conditions normal for lepidopteran larval midguts. Treatment of cells with chloroquine, a lysosomotropic agent, did not significantly affect PDV fusion to cells or infectivity in tissue culture assays.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-1097539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-1316456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-16346355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-1906550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-20517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-208249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-2154158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-2161350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-2173267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-2177356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-2184772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-2803002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-2820977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-2821175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-2833394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-2846352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-2916331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-3063616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-3107838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-3259178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-3622762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-362696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-3733744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-3905823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-4606365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-5044280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-6098295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-6292466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-6295978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-6310550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-6359230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-6370236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-6388149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-6398564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-6726888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-6991511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-7436739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-7463480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-787558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8445715-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1860-8
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:8445715-Animals,
pubmed-meshheading:8445715-Baculoviridae,
pubmed-meshheading:8445715-Binding Sites,
pubmed-meshheading:8445715-Cells, Cultured,
pubmed-meshheading:8445715-Chloroquine,
pubmed-meshheading:8445715-Endocytosis,
pubmed-meshheading:8445715-Glutaral,
pubmed-meshheading:8445715-Hydrogen-Ion Concentration,
pubmed-meshheading:8445715-Insects,
pubmed-meshheading:8445715-Kinetics,
pubmed-meshheading:8445715-Membrane Fusion,
pubmed-meshheading:8445715-Microvilli,
pubmed-meshheading:8445715-Receptors, Virus
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pubmed:year |
1993
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pubmed:articleTitle |
Saturable attachment sites for polyhedron-derived baculovirus on insect cells and evidence for entry via direct membrane fusion.
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pubmed:affiliation |
Department of Microbiology, University of Massachusetts, Amherst 01003.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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