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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1993-4-8
|
| pubmed:abstractText |
Functions of the tail region of neurofilament L have, to date, not been clearly elucidated. Bovine neurofilament L was cleaved into tail-less neurofilament L (50 kDa) and a tail fragment (19 kDa), by thrombin. Tail-less neurofilament L was deficit of the highly acidic domain of the tail region (approximately 77% of the entire region). Assembly of tail-less neurofilament L. was observed to be accelerated by both fluorometric and centrifugal measurements, compared with intact neurofilament L. The critical concentration of tail-less neurofilament L, which constitutes the constant unassembled pool, was approximately 0.25-times lower than that of neurofilament L. Under physiological conditions, tail-less neurofilament L formed a ribbon-like structure, whereas tail-less neurofilament L could form 10-nm filaments in an extremely low ionic-strength buffer in the presence of 1 mM MgCl2. An affinity-purified antibody directed against the tail fragment also accelerated neurofilament L assembly. The tail fragment neither coassembled with neurofilament L nor affect neurofilament L assembly. The acidic domain of the tail region may regulate neurofilament assembly and may be involved in 10-nm filament formation under physiological conditions.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0014-2956
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
212
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
565-71
|
| pubmed:dateRevised |
2007-7-23
|
| pubmed:meshHeading |
pubmed-meshheading:8444193-Amino Acid Sequence,
pubmed-meshheading:8444193-Animals,
pubmed-meshheading:8444193-Cattle,
pubmed-meshheading:8444193-Hydrogen-Ion Concentration,
pubmed-meshheading:8444193-Magnesium,
pubmed-meshheading:8444193-Microscopy, Electron,
pubmed-meshheading:8444193-Molecular Sequence Data,
pubmed-meshheading:8444193-Neurofilament Proteins,
pubmed-meshheading:8444193-Osmolar Concentration
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Acceleration of bovine neurofilament L assembly by deprivation of acidic tail domain.
|
| pubmed:affiliation |
Department of Neuropsychiatry, Osaka University Medical School, Fukushima-ku, Japan.
|
| pubmed:publicationType |
Journal Article
|