Linked Life Data

8443341

Source:http://linkedlifedata.com/resource/pubmed/id/8443341

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-4-6
pubmed:abstractText
Myrosinase isoenzymes are known to be encoded by two different families of genes denoted MA and MB. Nucleotide sequence analysis of a Brassica napus genomic clone containing a gene for myrosinase revealed it to be a pseudogene of the MA family. The gene spans more than 5 kb and contains at least 12 exons. The exon sequence of the gene is highly similar to myrosinase cDNA sequences. However, the gene displays three potential or actual pseudogene characters. Southern blot analysis using probes from the 3' portions of the genomic and B. napus MA and MB cDNA clones showed that MA type myrosinases are encoded by approximately 4 genes, while MB type myrosinases are encoded by more than 10 genes in B. napus. Northern blots with mRNA from seeds and young leaves probed with the MA- and MB-specific probes showed that the MA and MB myrosinase gene families are differentially expressed. Myrosinases are highly similar to proteins of a beta-glycosidase enzyme family comprising both beta-glycosidases and phospho-beta-glycosidases of as diverged species as archaebacteria, bacteria, mammals and plants. By homology to these beta-glycosidases, putative active site residues in myrosinase are discussed on the basis of the similarity between beta-glycosidases and cellulases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0167-4412
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
463-74
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Characterization of a Brassica napus myrosinase pseudogene: myrosinases are members of the BGA family of beta-glycosidases.
pubmed:affiliation
Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't