Linked Life Data

8443206

Source:http://linkedlifedata.com/resource/pubmed/id/8443206

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1 Pt 1
pubmed:dateCreated
1993-4-2
pubmed:abstractText
We have studied the effects of adding 0.5 mmol/L CaCl2 to University of Wisconsin solution (0.08 mmol/L free Ca++) on hypothermic heart preservation. Isolated pig hearts were subjected to 8 hours of preservation at 12 degrees C; eight hearts were arrested with Ca++ free University of Wisconsin solution, and seven hearts were arrested with Ca(++)-containing University of Wisconsin solution. The recovery of contractile function was evaluated by measuring isovolumic ventricular pressure development. 31P nuclear magnetic resonance spectroscopy was used to monitor the changes in high-energy phosphates. Compared to the hearts arrested with the Ca(++)-free University of Wisconsin solution, the heart arrested with the Ca(++)-containing University of Wisconsin solution showed significantly improved (p < 0.001) contractile functional recovery. No "stone heart" or loss of high-energy phosphates was observed on reperfusion. The hearts showed an increase in diastolic pressure during infusion of the Ca(++)-containing University of Wisconsin solution, however, to show the relationship between the addition of calcium and the increase in diastolic pressure, a second protocol was performed. A 30-minute period of ischemia was induced in thirteen hearts that were arrested at 12 degrees C with either Ca(++)-containing University of Wisconsin solution (n = 8) or Ca(++)-free University of Wisconsin solution (n = 5). Diastolic pressure was monitored during ischemia while ventricular volume was maintained constant with a balloon. The hearts arrested with the Ca(++)-containing University of Wisconsin solution showed a mean rise of 5 mm Hg in diastolic pressure and a rapid decline of phosphocreatine (p < 0.001). Our results suggest that, although 0.08 mmol/L free Ca++ improves functional recovery after 8 hours of heart preservation with University of Wisconsin solution at 12 degrees C, it can increase diastolic pressure during ischemia and accelerate breakdown of the high-energy phosphate stores in the myocardium, suggesting that use of University of Wisconsin solution containing 0.5 mmol/L CaCl2 may result in a significant increase in the intracellular calcium level.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1053-2498
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-8
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:8443206-Adenosine, pubmed-meshheading:8443206-Allopurinol, pubmed-meshheading:8443206-Animals, pubmed-meshheading:8443206-Calcium, pubmed-meshheading:8443206-Cardioplegic Solutions, pubmed-meshheading:8443206-Energy Metabolism, pubmed-meshheading:8443206-Glutathione, pubmed-meshheading:8443206-Heart, pubmed-meshheading:8443206-Heart Arrest, Induced, pubmed-meshheading:8443206-Insulin, pubmed-meshheading:8443206-Magnetic Resonance Spectroscopy, pubmed-meshheading:8443206-Myocardial Contraction, pubmed-meshheading:8443206-Myocardium, pubmed-meshheading:8443206-Organ Preservation Solutions, pubmed-meshheading:8443206-Phosphates, pubmed-meshheading:8443206-Raffinose, pubmed-meshheading:8443206-Solutions, pubmed-meshheading:8443206-Swine, pubmed-meshheading:8443206-Ventricular Function, Left
pubmed:articleTitle
The effects of Ca++ on the preservation of myocardial energy and function with University of Wisconsin solution. A 31P nuclear magnetic resonance study of isolated blood perfused Langendorff pig hearts.
pubmed:affiliation
Department of Physiology, Faculty of Medicine, University of Ottawa, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't