Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1993-4-1
|
| pubmed:abstractText |
An enzyme-linked immunosorbent assay using monoclonal antibodies to human lung surfactant protein A (SP-A) was applied to sera from patients with lung diseases. We examined whether SP-A appears in the sera of patients with diseases that are known to cause alterations in surfactant composition in bronchoalveolar lavage fluids, and we characterized the SP-A that was found. The level of SP-A in sera from 57 healthy volunteers was 45 +/- 3 ng/ml (mean +/- SEM). The levels in patients with idiopathic pulmonary fibrosis (IPF) (205 +/- 23 ng/ml, n = 32) and pulmonary alveolar proteinosis (PAP) (285 +/- 23 ng/ml, n = 6) were significantly higher than those in healthy control subjects (p < 0.01), whereas those of sarcoidosis (n = 16), pneumonia (n = 14), and tuberculosis (n = 14) were 52 +/- 27 ng/ml, 65 +/- 11 ng/ml, and 49 +/- 23 ng/ml, respectively. Electrophoresis and immunoblotting analysis demonstrated that the fraction isolated from serum of a patient with PAP or IPF by anti-SP-A immunoaffinity column chromatography consisted chiefly of human IgG and IgM, and that it also contained SP-A. Furthermore, IgG was found in preparation of purified human SP-A. SP-A was demonstrated to bind to nonimmune IgG coated onto microtiter wells. Gel filtration analysis revealed that serum SP-A was eluted at fractions of larger molecular size than was the purified SP-A. These findings suggest that SP-A appears in the bloodstream as a complex with immunoglobulin in IPF and in PAP.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactants
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0003-0805
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
147
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
723-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8442609-Aged,
pubmed-meshheading:8442609-Chronic Disease,
pubmed-meshheading:8442609-Glycoproteins,
pubmed-meshheading:8442609-Hepatitis,
pubmed-meshheading:8442609-Humans,
pubmed-meshheading:8442609-Liver Cirrhosis,
pubmed-meshheading:8442609-Lung Diseases,
pubmed-meshheading:8442609-Methods,
pubmed-meshheading:8442609-Middle Aged,
pubmed-meshheading:8442609-Pancreatitis,
pubmed-meshheading:8442609-Proteolipids,
pubmed-meshheading:8442609-Pulmonary Alveolar Proteinosis,
pubmed-meshheading:8442609-Pulmonary Fibrosis,
pubmed-meshheading:8442609-Pulmonary Surfactant-Associated Protein A,
pubmed-meshheading:8442609-Pulmonary Surfactant-Associated Proteins,
pubmed-meshheading:8442609-Pulmonary Surfactants,
pubmed-meshheading:8442609-Sarcoidosis,
pubmed-meshheading:8442609-Tuberculosis, Pulmonary
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Elevated levels of lung surfactant protein A in sera from patients with idiopathic pulmonary fibrosis and pulmonary alveolar proteinosis.
|
| pubmed:affiliation |
Department of Biochemistry, Sapporo Medical College, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|