Linked Life Data

8440521

Source:http://linkedlifedata.com/resource/pubmed/id/8440521

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-4-1
pubmed:abstractText
Methods following the process of binding and internalization of antibodies to cell surface antigens have often employed low pH isoosmolar buffers in order to dissociate surface antigen-antibody complexes. One of the most widely used buffers is a 0.05 M glycine-HCL buffer pH 2.8. Since the efficacy of action of this buffer was critical to a series of internalization experiments employing monoclonal antibodies (Mabs) to carcinoembryonic antigen (CEA) expressing cancer cell lines in this laboratory, we tested its performance in a number of different assays. Our results indicate that this buffer only partially dissociates antigen-antibody bonds and therefore can introduce major inaccuracies in internalization experiments.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0882-0139
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8440521-Antibodies, Monoclonal, pubmed-meshheading:8440521-Antibodies, Neoplasm, pubmed-meshheading:8440521-Antigen-Antibody Complex, pubmed-meshheading:8440521-Antigens, Neoplasm, pubmed-meshheading:8440521-Artifacts, pubmed-meshheading:8440521-Buffers, pubmed-meshheading:8440521-Carcinoembryonic Antigen, pubmed-meshheading:8440521-Endocytosis, pubmed-meshheading:8440521-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:8440521-Glycine, pubmed-meshheading:8440521-Humans, pubmed-meshheading:8440521-Hydrochloric Acid, pubmed-meshheading:8440521-Hydrogen-Ion Concentration, pubmed-meshheading:8440521-Immunoenzyme Techniques, pubmed-meshheading:8440521-Protein Binding, pubmed-meshheading:8440521-Reproducibility of Results, pubmed-meshheading:8440521-Tumor Cells, Cultured
pubmed:year
1993
pubmed:articleTitle
Cell membrane antigen-antibody complex dissociation by the widely used glycine-HCL method: an unreliable procedure for studying antibody internalization.
pubmed:affiliation
Oncology Research, Memorial University, St. John's, Newfoundland, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't