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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1993-3-31
pubmed:databankReference
pubmed:abstractText
Koningic acid inhibits glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by binding to the SH group in the active center. The fungus Trichoderma koningii, the producer of koningic acid, contains two GAPDH isozymes (GAPDHs I and II). GAPDH I is inhibited 50% by 1.1.10(-3) M koningic acid, while GAPDH II is inhibited 50% at 6.8 x 10(-6) M. cDNAs of the two isozymes were cloned from T. koningii and their nucleotide sequences were determined. The sequence of coding region and codon usage in both clones were compared with each other and with those of the gene for Aspergillus nidulans GAPDH (enzyme activity is inhibited 50% by 2.7 x 10(-7) M koningic acid). Results indicated that GAPDH II is more closely related to A. nidulans GAPDH than GAPDH I. All essential amino acid residues, except 174 and 181, which are implicated in catalysis and binding of NAD and substrates, were conserved among A. nidulans GAPDH and GAPDHs I and II. Residues 174 and 181 are threonine in both A. nidulans GAPDH and GAPDH II, but alanine and serine, respectively, in GAPDH I. The side-chain of alanine-174 in GAPDH I can not replace threonine-174 functionally as threonine-174 side-chain forms a hydrogen bond with the catalytically essential histidine-176.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
1172
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
43-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8439569-Amino Acid Sequence, pubmed-meshheading:8439569-Animals, pubmed-meshheading:8439569-Aspergillus nidulans, pubmed-meshheading:8439569-Base Sequence, pubmed-meshheading:8439569-Binding Sites, pubmed-meshheading:8439569-Cloning, Molecular, pubmed-meshheading:8439569-DNA, Fungal, pubmed-meshheading:8439569-Glyceraldehyde-3-Phosphate Dehydrogenases, pubmed-meshheading:8439569-Humans, pubmed-meshheading:8439569-Isoenzymes, pubmed-meshheading:8439569-Molecular Sequence Data, pubmed-meshheading:8439569-RNA, Messenger, pubmed-meshheading:8439569-Recombinant Proteins, pubmed-meshheading:8439569-Restriction Mapping, pubmed-meshheading:8439569-Sequence Homology, Amino Acid, pubmed-meshheading:8439569-Sequence Homology, Nucleic Acid, pubmed-meshheading:8439569-Sesquiterpenes, pubmed-meshheading:8439569-Trichoderma
pubmed:year
1993
pubmed:articleTitle
Cloning of two isozymes of Trichoderma koningii glyceraldehyde-3-phosphate dehydrogenase with different sensitivity to koningic acid.
pubmed:affiliation
Department of Applied Biological Science, Tokyo Noko University, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't