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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1993-3-31
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pubmed:databankReference |
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pubmed:abstractText |
Southern analysis of the human genome revealed that there are several sequences with homology to the nonhistone chromosomal protein HMG-1. The majority of the HMG-1 sequences are intronless as suggested from the polymerase chain reaction of HeLa DNA. Sequencing of a clone from a human placenta genomic library revealed that the clone was intronless and displayed 99% homology to the human HMG-1 cDNA. The 5' regulatory motif, CCAAT, is present in the clone but there is no TATA-box. Most of the differences between the HMG-1 cDNA sequence and the clone involve point mutations with no interruption of the reading frame. The sequence is flanked at 5' and 3' ends by a 15 nucleotide long direct repeat suggesting that the clone is a processed HMG-1 retropseudogene. Sequence differences between the reading frames of the HMG-1 pseudogene and HMG-1 cDNA indicated that the pseudogene arose relatively late in evolution, approximately one million years ago. The present paper is the first study on a genomic sequence related to HMG-1 genes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
1172
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
231-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8439568-Amino Acid Sequence,
pubmed-meshheading:8439568-Base Sequence,
pubmed-meshheading:8439568-Blotting, Southern,
pubmed-meshheading:8439568-Cloning, Molecular,
pubmed-meshheading:8439568-DNA,
pubmed-meshheading:8439568-Female,
pubmed-meshheading:8439568-Genome, Human,
pubmed-meshheading:8439568-HeLa Cells,
pubmed-meshheading:8439568-High Mobility Group Proteins,
pubmed-meshheading:8439568-Humans,
pubmed-meshheading:8439568-Molecular Sequence Data,
pubmed-meshheading:8439568-Placenta,
pubmed-meshheading:8439568-Point Mutation,
pubmed-meshheading:8439568-Polymerase Chain Reaction,
pubmed-meshheading:8439568-Pregnancy,
pubmed-meshheading:8439568-Pseudogenes,
pubmed-meshheading:8439568-Reading Frames,
pubmed-meshheading:8439568-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8439568-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:8439568-Restriction Mapping,
pubmed-meshheading:8439568-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8439568-TATA Box
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pubmed:year |
1993
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pubmed:articleTitle |
A retropseudogene for non-histone chromosomal protein HMG-1.
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pubmed:affiliation |
Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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