| pubmed-article:8436597 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8436597 | lifeskim:mentions | umls-concept:C0021753 | lld:lifeskim |
| pubmed-article:8436597 | lifeskim:mentions | umls-concept:C2697656 | lld:lifeskim |
| pubmed-article:8436597 | lifeskim:mentions | umls-concept:C0027950 | lld:lifeskim |
| pubmed-article:8436597 | lifeskim:mentions | umls-concept:C0175697 | lld:lifeskim |
| pubmed-article:8436597 | lifeskim:mentions | umls-concept:C1159339 | lld:lifeskim |
| pubmed-article:8436597 | lifeskim:mentions | umls-concept:C0023810 | lld:lifeskim |
| pubmed-article:8436597 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:8436597 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:8436597 | lifeskim:mentions | umls-concept:C1366571 | lld:lifeskim |
| pubmed-article:8436597 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:8436597 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8436597 | pubmed:dateCreated | 1993-3-23 | lld:pubmed |
| pubmed-article:8436597 | pubmed:abstractText | Neutrophils are possibly involved in the pathogenesis of various lung diseases through the release of numerous mediators. In the present study, we studied the regulation of IL-8 gene induction and protein secretion in human blood neutrophils. Northern blot analysis revealed that LPS increased IL-8 mRNA levels in neutrophils, with a maximal fivefold increase by 2 h. IL-8 mRNa levels returned to baseline values within 12 h. In contrast, LPS-stimulated monocytes demonstrated a sustained increase of IL-8 mRNA levels for more than 24 h. TNF-alpha, IL-1 beta, and phorbol myristate acetate also increased IL-8 mRNA levels in neutrophils. Immunohistochemical analysis confirmed that IL-8 was localized within stimulated neutrophils. IL-8 secretion by neutrophils and monocytes was quantified using a specific ELISA for IL-8. Resting neutrophils secreted minimal IL-8 activity. However when cells were stimulated with LPS, TNF-alpha, or IL-1B, neutrophils secreted IL-8. IL-8 secretion was most marked during the first 2 h after stimulation and decreased thereafter. In contrast, monocytes maintained a high rate of IL-8 secretion over 12 h. Although a single monocyte secreted 70-fold more IL-8 than did a single neutrophil after 4 h of incubation, the high abundance of neutrophils in peripheral blood made the neutrophil-secreted IL-8 more significant. During the first 2 h, neutrophils secreted approximately 40% of the IL-8 released by monocytes in the same volume of blood. This ratio decreased to 9% after 12 h. Neutrophil-secreted IL-8 may play an autocrine or paracrine role during the initial stage of inflammation. | lld:pubmed |
| pubmed-article:8436597 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8436597 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8436597 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8436597 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8436597 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:8436597 | pubmed:issn | 0021-9541 | lld:pubmed |
| pubmed-article:8436597 | pubmed:author | pubmed-author:KimYY | lld:pubmed |
| pubmed-article:8436597 | pubmed:author | pubmed-author:HamL BLB | lld:pubmed |
| pubmed-article:8436597 | pubmed:author | pubmed-author:LumL CLC | lld:pubmed |
| pubmed-article:8436597 | pubmed:author | pubmed-author:LarrickJ WJW | lld:pubmed |
| pubmed-article:8436597 | pubmed:author | pubmed-author:RaffinT ATA | lld:pubmed |
| pubmed-article:8436597 | pubmed:author | pubmed-author:DanielDD | lld:pubmed |
| pubmed-article:8436597 | pubmed:author | pubmed-author:WallaceE FEF | lld:pubmed |
| pubmed-article:8436597 | pubmed:author | pubmed-author:FujishimaSS | lld:pubmed |
| pubmed-article:8436597 | pubmed:author | pubmed-author:HoffmanA RAR | lld:pubmed |
| pubmed-article:8436597 | pubmed:author | pubmed-author:ZhengHH | lld:pubmed |
| pubmed-article:8436597 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8436597 | pubmed:volume | 154 | lld:pubmed |
| pubmed-article:8436597 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8436597 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8436597 | pubmed:pagination | 478-85 | lld:pubmed |
| pubmed-article:8436597 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:8436597 | pubmed:meshHeading | pubmed-meshheading:8436597-... | lld:pubmed |
| pubmed-article:8436597 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8436597 | pubmed:articleTitle | Regulation of neutrophil interleukin 8 gene expression and protein secretion by LPS, TNF-alpha, and IL-1 beta. | lld:pubmed |
| pubmed-article:8436597 | pubmed:affiliation | Department of Medicine Stanford University Medical Center, California 94305-5236. | lld:pubmed |
| pubmed-article:8436597 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8436597 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:8436597 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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