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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-3-12
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pubmed:abstractText |
The lack of HLA class I antigen expression by the melanoma cell line SK-MEL-33 is caused by a unique lesion in beta 2-microglobulin (beta 2-mu). Sequencing of beta 2-mu mRNA detected a guanosine deletion at position 323 in codon 76 that causes a frameshift with a subsequent introduction of a stop codon at a position 54 base upstream of the normal position of the stop codon in the message. The loss of 18 amino acids and the change of 6 amino acids, including a cysteine at position 80 in the carboxy terminus of beta 2-mu, are likely to cause marked changes in the structure of the polypeptide. The latter may account for the inability of beta 2-mu to associate with HLA class I heavy chains and for its lack of reactivity with the anti-beta 2-mu mAb tested. HLA class I antigen expression on SK-MEL-33 cells was reconstituted after transfection with a wild-type B2m gene, therefore indicating that the abnormality of endogenous B2m gene is the only mechanism underlying lack of HLA class I antigen expression by SK-MEL-33 cells. The guanosine deletion in B2m gene was detected also in the melanoma tissue from which SK-MEL-33 cells had originated. Therefore, the molecular lesion identified in the SK-MEL-33 melanoma cell line is not caused by a mutation acquired during growth in vitro but is likely to reflect a somatic mutation during tumor progression.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-11894933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-1898655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-1912517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-2101727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-2146210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-2201309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-2259384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-2326874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-2411790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-2660144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-2699240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-2784406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-2826637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-2840197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-2999980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-3012568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-3023861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-3114385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-3309677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-3312414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-3338074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-3458188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-3474623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-3514206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-3906658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-4121426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-4139784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-46595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-4850204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-53197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-6086763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-6165997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-6165999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-6171820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-6180834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-6181590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-6186604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-6190070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-6329026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-637870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-667938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-6966655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-7016338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-87477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8432869-91522
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
684-92
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8432869-Aged,
pubmed-meshheading:8432869-Base Sequence,
pubmed-meshheading:8432869-Frameshift Mutation,
pubmed-meshheading:8432869-Gene Deletion,
pubmed-meshheading:8432869-Histocompatibility Antigens Class I,
pubmed-meshheading:8432869-Humans,
pubmed-meshheading:8432869-Male,
pubmed-meshheading:8432869-Melanoma,
pubmed-meshheading:8432869-Molecular Sequence Data,
pubmed-meshheading:8432869-RNA, Messenger,
pubmed-meshheading:8432869-Transfection,
pubmed-meshheading:8432869-Tumor Cells, Cultured,
pubmed-meshheading:8432869-beta 2-Microglobulin
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pubmed:year |
1993
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pubmed:articleTitle |
Lack of HLA class I antigen expression by melanoma cells SK-MEL-33 caused by a reading frameshift in beta 2-microglobulin messenger RNA.
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pubmed:affiliation |
Department of Microbiology and Immunology, New York Medical College, Valhalla 10595.
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