| pubmed-article:8432811 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8432811 | lifeskim:mentions | umls-concept:C0042776 | lld:lifeskim |
| pubmed-article:8432811 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8432811 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:8432811 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:8432811 | lifeskim:mentions | umls-concept:C0750484 | lld:lifeskim |
| pubmed-article:8432811 | lifeskim:mentions | umls-concept:C0220825 | lld:lifeskim |
| pubmed-article:8432811 | lifeskim:mentions | umls-concept:C0201773 | lld:lifeskim |
| pubmed-article:8432811 | lifeskim:mentions | umls-concept:C0205473 | lld:lifeskim |
| pubmed-article:8432811 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:8432811 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8432811 | pubmed:dateCreated | 1993-3-16 | lld:pubmed |
| pubmed-article:8432811 | pubmed:abstractText | The confirmation of infection with human T-cell lymphotropic virus type I (HTLV-I) and type II (HTLV-II) currently involves multiple assays. These include Western blot (immunoblot) (WB) and/or radioimmunoprecipitation assay for detection of antibodies to HTLV-specific viral proteins and polymerase chain reaction and/or peptide-based enzyme immunoassays for differentiating between the two viruses. We undertook an evaluation of a modified WB assay that includes native HTLV-I viral proteins from MT-2 cells spiked with an HTLV-I transmembrane glycoprotein (recombinant p21e) and the HTLV-I- and HTLV-II-specific recombinant proteins MTA-1 and K55. The test panel consisted of well-characterized sera from U.S. blood donors, American Indians, intravenous drug users, and patients seen in sexually transmitted disease clinics. Of 158 HTLV-I/II-seropositive serum specimens tested, 156 (98.7%) were confirmed and typed as HTLV-I or HTLV-II. Of 82 HTLV-I/II-seroindeterminate or -seronegative serum specimens, only 1 was classified as HTLV-II positive: the sample had weak gag p19 and strong gag p24 and env p21e reactivity and was radioimmunoprecipitation assay negative for env gp61/68 but polymerase chain reaction positive for HTLV-II. The specificity of the modified WB for confirming and typing serum samples was therefore 100%. We conclude that this WB assay is useful for confirming and typing HTLV infection and can help simplify HTLV-I/II testing algorithms. | lld:pubmed |
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| pubmed-article:8432811 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8432811 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8432811 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8432811 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8432811 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8432811 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8432811 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:8432811 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:8432811 | pubmed:author | pubmed-author:KaplanJ EJE | lld:pubmed |
| pubmed-article:8432811 | pubmed:author | pubmed-author:ChanLL | lld:pubmed |
| pubmed-article:8432811 | pubmed:author | pubmed-author:RobertsB DBD | lld:pubmed |
| pubmed-article:8432811 | pubmed:author | pubmed-author:LipkaJ JJJ | lld:pubmed |
| pubmed-article:8432811 | pubmed:author | pubmed-author:ReyesG RGR | lld:pubmed |
| pubmed-article:8432811 | pubmed:author | pubmed-author:FoungS KSK | lld:pubmed |
| pubmed-article:8432811 | pubmed:author | pubmed-author:KhabbazR FRF | lld:pubmed |
| pubmed-article:8432811 | pubmed:author | pubmed-author:HeneineWW | lld:pubmed |
| pubmed-article:8432811 | pubmed:author | pubmed-author:HadlockK GKG | lld:pubmed |
| pubmed-article:8432811 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8432811 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:8432811 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8432811 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8432811 | pubmed:pagination | 260-4 | lld:pubmed |
| pubmed-article:8432811 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:8432811 | pubmed:meshHeading | pubmed-meshheading:8432811-... | lld:pubmed |
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| pubmed-article:8432811 | pubmed:meshHeading | pubmed-meshheading:8432811-... | lld:pubmed |
| pubmed-article:8432811 | pubmed:meshHeading | pubmed-meshheading:8432811-... | lld:pubmed |
| pubmed-article:8432811 | pubmed:meshHeading | pubmed-meshheading:8432811-... | lld:pubmed |
| pubmed-article:8432811 | pubmed:meshHeading | pubmed-meshheading:8432811-... | lld:pubmed |
| pubmed-article:8432811 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8432811 | pubmed:articleTitle | Evaluation of an immunoblot assay for serological confirmation and differentiation of human T-cell lymphotropic virus types I and II. | lld:pubmed |
| pubmed-article:8432811 | pubmed:affiliation | Division of Viral and Rickettsial Diseases, Centers for Disease Control, Atlanta, Georgia 30333. | lld:pubmed |
| pubmed-article:8432811 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8432811 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8432811 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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